Extracellular glutamate and other metabolites in and around RG2 rat glioma: an intracerebral microdialysis study
Extracellular glutamate and other metabolites in and around RG2 rat glioma: an intracerebral microdialysis study
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DOI：
10.1023/a:1006426917654
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发表时间：
2000-03-01
影响因子：
3.9
通讯作者：
Henning, J
中科院分区：
文献类型：
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作者：
Behrens, PF;Langemann, H;Henning, J
本研究测定了恶性胶质瘤啮齿动物模型、瘤周间隙和邻近皮质中谷氨酸、10种额外氨基酸、乳酸、葡萄糖和一些抗氧化剂的细胞外含量。通过标准化程序在Fischer-344大鼠（n = 10）的右额叶皮质中诱导RG 2肿瘤，以获得3-4 mm直径的最大矢状肿瘤宽度。在通过对比增强MRI确认肿瘤生长和定位后，将三个微透析探针同时植入皮质中：在肿瘤植入部位（肿瘤），尾部2 mm，肿瘤周围脑（BAT）和尾部4 mm（皮质）到植入部位。与皮质相比，肿瘤中谷氨酸的透析液浓度增加3.9倍，BAT中增加2倍。甘氨酸在肿瘤中升高11.4倍，在BAT中升高2.6倍。肿瘤中的乳酸盐增加了1.7倍，BAT中增加了1.2倍。葡萄糖、抗坏血酸和尿酸在肿瘤、BAT和皮质中无显著性差异。肿瘤周围间隙中谷氨酸盐和甘氨酸的透析液水平升高可能导致与人类这种肿瘤类型相关的神经元功能受损和癫痫样活动。
The current study determined the extracellular content of glutamate, 10 additional amino acids, lactate, glucose and some antioxidants in a rodent model of malignant glioma, its peritumoral space and the adjacent cortex. RG2 tumors were induced in the right frontal cortex of Fischer-344 rats (n = 10) by a standardized procedure to obtain a maximum sagittal tumor width of 3-4 mm diameter. After confirmation of tumor growth and localization by contrast enhanced MRI three microdialysis probes were implanted simultaneously in the cortex: at the tumor implantation site (tumor), 2 mm caudally, brain around tumor (BAT) and 4 mm caudally (cortex) to the site of implantation. Dialysate concentrations of glutamate were increased 3.9-fold in tumor and 2-fold in BAT compared with cortex. Glycine was elevated 11.4-fold in tumor and 2.6-fold in BAT. Lactate was increased 1.7-fold in tumor, 1.2-fold in BAT. Levels of glucose, ascorbic acid and uric acid were not significantly different in tumor, BAT and cortex. The increased dialysate levels of glutamate and glycine in the peritumoral space may contribute to impaired neuronal function and epileptiform activity associated with this tumor type in humans.