Multiphoton fluorescence lifetime imaging of intrinsic fluorescence in human and rat brain tissue reveals spatially distinct NADH binding
Multiphoton fluorescence lifetime imaging of intrinsic fluorescence in human and rat brain tissue reveals spatially distinct NADH binding
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DOI：
10.1364/oe.16.004237
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发表时间：
2008-03-17
期刊：
影响因子：
3.8
通讯作者：
Levene, Michael J.
中科院分区：
文献类型：
--
作者：
Chia, Thomas H.;Williamson, Anne;Levene, Michael J.
分子的双光子荧光寿命成像（FLIM）可以揭示局部微环境的重要信息。NADH是一种内在的荧光分子和普遍存在的代谢辅酶，其寿命强烈依赖于酶结合。我们提出了一个自定义的图像处理算法的原始荧光寿命和振幅数据，产生的图像显示空间上不同的NADH荧光寿命在大鼠和人脑切片。在对照和癫痫大鼠组织中收集NADH FLIM图像。NADH寿命的空间模式的差异支持的假设，即NADH结合，从而代谢能力，是显着不同的群体之间。这种类型的分析可以提供关于病理材料中的代谢状态的信息。(c)2008年美国光学学会。
Two-photon fluorescence lifetime imaging ( FLIM) of molecules can reveal important information on the local microenvironment. NADH, an intrinsic fluorescent molecule and ubiquitous metabolic co-enzyme, has a lifetime that depends strongly on enzymatic binding. We present a custom image-processing algorithm for raw fluorescence lifetime and amplitude data that produces an image showing spatially distinct NADH fluorescence lifetimes in slices of rat and human brain. NADH FLIM images were collected in control and epileptic rat tissue. Differences in spatial patterns of NADH lifetimes support the hypothesis that NADH binding, and thus metabolic capacity, is significantly different between groups. This type of analysis can provide information on metabolic states in pathological material. (c) 2008 Optical Society of America.