16S rRNA metagenomic analysis of the symbiotic community structures of bacteria in foregut, midgut, and hindgut of the wood-feeding termite Bulbitermes sp.
16S rRNA metagenomic analysis of the symbiotic community structures of bacteria in foregut, midgut, and hindgut of the wood-feeding termite Bulbitermes sp.
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DOI：
10.1007/s13199-018-0544-5
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发表时间：
2018-10-01
期刊：
影响因子：
2.5
通讯作者：
Yahya, Adibah
中科院分区：
文献类型：
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作者：
Chew, Yue Ming;Lye, SiewFen;Yahya, Adibah
白蚁肠道是一个高度结构化的微生境，具有不同的物理化学区域。一般分为前肠、中肠和后肠。肠道内不同的物理化学条件对肠道微生物区系的分布有很大影响。因此，每个肠道隔间都有一个独特的微生物种群结构。本研究对食材高等白蚁Bulbitermes sp.的前肠、中肠和后肠的细菌群落进行了研究。通过16S rRNA V3-V4区的元基因组测序，对其进行了详细分析。前肠和中肠的微生物群具有相似的分类模式，而后肠具有更多不同的细菌系统类型。前肠和中肠的群落由芽孢杆菌和梭状芽孢杆菌以及放线菌分类群(放线杆菌)组成。在后肠中发现的主要细菌谱系是螺旋毛菌科(螺旋毛纲)。三种肠道之间的显著差异是前肠和中肠中潜在的木质素降解菌放线菌的相对丰度。这表明木质素的修饰可能发生在白蚁肠道的前部。利用16S rRNA标记基因预测后基因组的功能表明，细胞运动、能量代谢以及辅因子和维生素的代谢主要发生在后肠微生物区，而外源物质的降解和代谢主要发生在前肠部分。这与我们的16S rRNA元基因组学结果相一致，该结果表明木质纤维素的降解过程是由木质素破坏启动的，增加了纤维素和半纤维素的可及性。
The termite gut is a highly structured microhabitat with physicochemically distinct regions. It is generally separated into the foregut, midgut and hindgut. The distribution of gut microbiota is greatly influenced by varying physicochemical conditions within the gut. Thus, each gut compartment has a unique microbial population structure. In this study, the bacterial communities of foregut, midgut and hindgut of wood-feeding higher termite, Bulbitermes sp. were analyzed in detail via metagenomic sequencing of the 16S rRNA V3-V4 region. While the microbiomes of the foregut and midgut shared a similar taxonomic pattern, the hindgut possessed more diverse bacterial phylotypes. The communities in the foregut and midgut were dominated by members of the group Bacilli and Clostridia (Firmicutes) as well as taxon Actinomycetales (Actinobacteria). The main bacterial lineage found in hindgut was Spirochaetaceae (Spirochaetes). The significant difference among the three guts was the relative abundance of the potential lignin-degrading bacteria, Actinomycetales, in both the foregut and midgut. This suggests that lignin modification was probably held in the anterior part of termite gut. Predictive functional profiles of the metagenomes using 16S rRNA marker gene showed that cell motility, energy metabolism and metabolism of cofactors and vitamins were found predominantly in hindgut microbiota, whereas xenobiotics degradation and metabolism mostly occurred in the foregut segment. This was compatible with our 16S rRNA metagenomic results showing that the lignocellulose degradation process was initiated by lignin disruption, increasing the accessibility of celluloses and hemicelluloses.