CEACAM1 inhibits cell-matrix adhesion and promotes cell migration through regulating the expression of N-cadherin
CEACAM1 inhibits cell-matrix adhesion and promotes cell migration through regulating the expression of N-cadherin
复制标题
DOI：
10.1016/j.bbrc.2012.11.107
复制
发表时间：
2013-01-11
影响因子：
3.1
通讯作者：
Duan, Haifeng
中科院分区：
文献类型：
--
作者：
Liu, Jin;Di, Guohu;Duan, Haifeng
癌胚抗原相关细胞粘附分子1（CEACAM 1）是免疫球蛋白超家族的一员，具有两种相互矛盾的功能：抑制肿瘤和促进肿瘤侵袭。在本研究中，我们发现CEACAM 1作为粘附抑制剂和迁移促进剂发挥作用。转染CEACAM 1的细胞，无论是293-CEACAM 1还是LOVO/trans-CEACAM 1，其粘附率均较低。此外，HT 29/siRNA-CEACAM 1细胞具有比HT 29细胞更高的粘附率。这些结果表明CEACAM 1是细胞-基质粘附的抑制剂。此外，293-CEACAM 1 LOVO/trans-CEACAM 1细胞在跨孔迁移测定中表现出更好的运动性。在293-CEACAM 1、LOVO/trans-CEACAM 1和HT 29/siRNA-CEACAM 1细胞中，N-cadherin表达水平与CEACAM 1呈正相关。当被GC-4抗体阻断后，293-CEACAM 1和LOVO/trans-CEACAM 1的粘附能力恢复，运动性受到抑制，表明CEACAM 1通过N-钙粘蛋白发挥作用。(C)2012 Elsevier Inc. All rights reserved.
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a member of the immunoglobulin super family and has been observed to have two paradoxical functions: tumor suppression and the promotion of tumor invasion. In the present study, we discovered that CEACAM1 functions as an adhesion inhibitor and a migration promoter. The CEACAM1 transfected cells, either 293-CEACAM1 or LOVO/trans-CEACAM1, was proved to have lower adhesion rate. Furthermore, HT29/siRNA-CEACAM1 cells had a higher adhesion rate than HT29 cells. These results indicated that CEACAM1 was an inhibitor of cell-matrix adhesion. Additionally, 293-CEACAM1 LOVO/trans-CEACAM1 cells exhibited better motility in a trans-well migration assay. N-cadherin expression levels were positively correlated with CEACAM1 in 293-CEACAM1, LOVO/trans-CEACAM1 and HT29/siRNA-CEACAM1 cells. When blocked by a GC-4 antibody, the adhesive capacities of 293-CEACAM1 and LOVO/trans-CEACAM1 were recovered and the motilities of them were suppressed, which suggested that CEACAM1 functioned through N-cadherin. (C) 2012 Elsevier Inc. All rights reserved.