Ultra-sensitive quantification of lysozyme based on element chelate labeling and capillary electrophoresis-inductively coupled plasma mass spectrometry.
Ultra-sensitive quantification of lysozyme based on element chelate labeling and capillary electrophoresis-inductively coupled plasma mass spectrometry.
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DOI：
10.1016/j.aca.2014.01.003
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发表时间：
2014-02
影响因子：
6.2
通讯作者：
Mingwei Yang;Weihua Wu;Yajuan Ruan;Limei Huang;Zujian Wu;Yong Cai;Fengfu Fu
中科院分区：
文献类型：
--
作者：
Mingwei Yang;Weihua Wu;Yajuan Ruan;Limei Huang;Zujian Wu;Yong Cai;Fengfu Fu
在本研究中，描述了一种基于 Gd3+二亚乙基三胺-N,N,N',N",N"-五乙酸标记和毛细管电泳-电感耦合等离子体质谱 (CE-ICP-MS) 的超灵敏溶菌酶定量方法。 Gd3+ 标记的溶菌酶可通过毛细管电泳 (CE) 有效分离，并通过电感耦合等离子体质谱 (ICP-MS) 灵敏地测定。基于钆标记和 CE-ICP-MS，可在 12 分钟内测定溶菌酶，检测限极低，为 3.89 attomole（100 nL 样品注射为 3.89 × 10−11mol L−1），RSD < 6% (n= 5)。该方法已成功用于检测唾液样本中的溶菌酶，回收率为 91-106%，表明我们的方法灵敏可靠。本方法的成功为低丰度蛋白质的生物测定和灵敏检测提供了新的潜力。
In this study, an ultra-sensitive method for the quantification of lysozyme based on the Gd3+diethylenetriamine-N,N,N′,N″,N″-pentaacetic acid labeling and capillary electrophoresis–inductively coupled plasma mass spectrometry (CE–ICP–MS) was described. The Gd3+-tagged lysozyme was effectively separated by capillary electrophoresis (CE) and sensitively determined by inductively coupled plasma mass spectrometry (ICP–MS). Based on the gadolinium-tagging and CE–ICP–MS, the lysozyme was determined within 12 min with an extremely low detection limit of 3.89 attomole (3.89 × 10−11mol L−1for 100 nL of sample injection) and a RSD < 6% (n= 5). The proposed method has been successfully used to detect lysozyme in saliva samples with a recovery of 91–106%, suggesting that our method is sensitive and reliable. The success of the present method provides a new potential for the biological assays and sensitive detection of low-abundant proteins.