Isolation of three crystalline proteins from beef liver after partial purification by acetone fractionation.
Isolation of three crystalline proteins from beef liver after partial purification by acetone fractionation.
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通过丙酮分级分离部分纯化后从牛肝中分离出三种结晶蛋白。
DOI：
10.1080/00327488108065539
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
G. Mourtzikos
中科院分区：
文献类型：
--
作者：
A. L. Dounce;G. Mourtzikos
描述了从碎牛肝中分离三种晶体形式的蛋白质。这些蛋白质是 FTBL 蛋白（Arch. Biochem. Biophys. 188, 251-265 (1978)、巴豆酸酶和过氧化氢酶。巴豆酸酶是通过从磨碎的肝脏的 32% 丙酮提取物中结晶分离的。FTBL 蛋白和过氧化氢酶随后可以从相同的提取物中分离。为了获得最佳产量和易于分离，FTBL 蛋白从 46.5% 丙酮提取物中分离，其中随后可以通过透析来结晶 FTBL 蛋白以及过氧化氢酶的分离，在实现结晶之前可以用异丙醇代替丙酮，并且对于过氧化氢酶，提出了蛋白质重结晶的方法，并讨论了有机溶剂在重结晶中的作用。
The isolation of three proteins in crystalline form from ground beef liver is described. These proteins are FTBL protein (Arch. Biochem. Biophys. 188, 251-265 (1978), crotonase, and catalase. Crotonase is isolated by crystallization from a 32% acetone extract of the ground liver. FTBL protein and catalase can subsequently be isolated from the same extract. For optimal yield and ease of isolation, FTBL protein is isolated from a 46.5% acetone extract from which catalase can subsequently be crystallized by dialysis. The isolation of FTBL protein as well as the isolation of catalase involves a preliminary fractional precipitation and solution before crystallization can be achieved. Isopropanol can be substituted for acetone in the isolation of the above three proteins and in the case of catalase, results in an exceptionally high yield. Methods for the recrystallization of the proteins are presented and the role of organic solvents in recrystallization is discussed.