Determinants of insulin concentrations in healthy 1-week-old babies in the community: Applications of a bloodspot assay
Determinants of insulin concentrations in healthy 1-week-old babies in the community: Applications of a bloodspot assay
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DOI：
10.1016/j.earlhumdev.2005.05.006
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发表时间：
2006-02-01
影响因子：
2.5
通讯作者：
Hattersley, AT
中科院分区：
文献类型：
--
作者：
Shields, BM;Knight, B;Hattersley, AT
背景资料：如果能够提高对新生儿期大量健康婴儿的胰岛素浓度进行测量的能力，将有助于对胰岛素分泌和胰岛素作用进行流行病学研究。目的：我们评估了一种可以测量分娩后7天常规古特里测试中采集的血斑中胰岛素的测定方法的使用。研究设计和受试者：对366名7日龄婴儿的足跟血点进行胰岛素和葡萄糖测定。结果：正常7日龄婴儿血斑胰岛素浓度（中位数（IQR）：15.4 pmol/l（< 10-28.5））远低于成年配偶空腹胰岛素浓度（44.3 pmol/l）。（30.6-72.6））（p < 0.001）。胰岛素和葡萄糖浓度相关（r=0.33，p < 0.001）。胰岛素和葡萄糖随进食时间显著下降。与母乳喂养的婴儿相比，奶瓶喂养的婴儿胰岛素浓度较高，但葡萄糖浓度相似。详细的分析，以解释混杂因素是有限的，由于时间的偏态分布，因为饲料和塔限制的测定导致非连续的insulin data.Conclusions：在最大的研究正常7天大的儿童到目前为止，我们已经表明胰岛素浓度较低，与成人和葡萄糖不同，从饲料的时间，和类型的饲料。这验证了使用血斑胰岛素测定作为大规模流行病学研究的潜在研究工具。然而，在未来的使用中需要仔细的研究设计，以减少由喂食时间和类型引起的变化以及三分之一的值处于或低于本试验下限的问题。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
Background: Epidemiological research into insulin secretion and insulin action would be helped by improved ability to measure insulin concentrations in large groups of healthy babies in the neonatal period. Such research is often restricted by the invasive nature of blood samplingAims: We assessed the use of an assay that can measure insulin from bloodspots taken during routine Guthrie testing 7 days after delivery. Study design and subjects: Insulin and glucose were measured in 366 seven-day-old infants from heel-prick bloodspots. Time since last feed and type of feed were recorded.Results: Bloodspot insulin concentrations in normal 7-day-old infants were much tower (median (IQR): 15.4 pmol/l (< 10-28.5)) than fasting insulin concentrations in adult mates (44.3 pmol/l. (30.6-72.6)) (p < 0.001). Insulin and glucose concentrations were correlated (r=0.33, p < 0.001). Insulin and glucose fell significantly with time from feed. Bottle fed infants had higher insulin concentrations but similar glucose concentrations compared to breast fed infants. Detailed analysis to account for confounders was limited due to the skewed distribution of time since feed and the tower limit of the assay Leading to non-continuous insulin data.Conclusions: In the largest study of normal 7-day-old children to date we have shown insulin concentrations are low compared to adults and vary with glucose, time from feed, and type of feed. This validates the use of the bloodspot insulin assay as a potential research toot for large-scale epidemiological studies. However, careful study design would be required in future use to reduce the variation caused by timing and type of feeding and the problem of one third of values being at or below the lower limit of this assay. (c) 2005 Elsevier Ireland Ltd. All rights reserved.