Nuclear localization and DNA interaction of protein disulfide isomerase ERp57 in mammalian cells
Nuclear localization and DNA interaction of protein disulfide isomerase ERp57 in mammalian cells
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DOI：
10.1002/jcb.10137
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发表时间：
2002-01-01
影响因子：
4
通讯作者：
Turano, C
中科院分区：
文献类型：
--
作者：
Coppari, S;Altieri, F;Turano, C
蛋白质二硫键异构酶ERp 57主要定位于内质网中，但也存在于细胞质中，根据初步证据，存在于禽类细胞的细胞核中。结论性的证据，其核定位和其在体内与DNA在哺乳动物细胞中的相互作用，在这里提供的基础上进行的DNA-蛋白质交联实验与两种不同的交联剂对活的HeLa和3 T3细胞。核ERp 57也可以检测到免疫荧光在HeLa细胞中，它显示出细胞内的分布明显不同的同源蛋白质，专门位于内质网。哺乳动物ERp 57在识别S/MAR样DNA序列和与核基质结合方面与禽类蛋白相似。可以假设，已知与其他蛋白质，特别是STAT 3和钙网蛋白相关的ERp 57可能有助于它们的核输入、DNA结合或它们在核内履行的其他功能。J.细胞。85：325-333，2002. (C)2002 Wiley-Liss，Inc.
Protein disulfide isomerase ERp57 is localized predominantly in the endoplasmic reticulum, but is also present in the cytosol and, according to preliminary evidence, in the nucleus of avian cells. Conclusive evidence of its nuclear localization and of its interaction with DNA in vivo in mammalian cells is provided here on the basis of DNA-protein cross-linking experiments performed with two different cross-linking agents on viable HeLa and 3T3 cells. Nuclear ERp57 could also be detected by immunofluorescence in HeLa cells, where it showed an intracellular distribution clearly different from that of an homologous protein, located exclusively in the endoplasmic reticulum. Mammalian ERp57 resembles the avian protein in its recognition of S/MAR-like DNA sequences and in its association with the nuclear matrix. It can be hypothesized that ERp57, which is known to associate with other proteins, in particular STAT3 and calreticulin, may contribute to their nuclear import, DNA binding, or other functions that they fulfil inside the nucleus. J. Cell. Biochem. 85: 325-333, 2002. (C) 2002 Wiley-Liss, Inc.