Formation of 9-hydroxy linoleic acid as a product of phospholipid peroxidation in diabetic erythrocyte membranes
Formation of 9-hydroxy linoleic acid as a product of phospholipid peroxidation in diabetic erythrocyte membranes
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DOI：
10.1016/s1388-1981(99)00052-9
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发表时间：
1999-05-18
影响因子：
4.8
通讯作者：
Sumino, K
中科院分区：
文献类型：
--
作者：
Inouye, M;Mio, T;Sumino, K
氧源性自由基（OFR）和脂质过氧化的增加可能导致糖尿病血管并发症。一些脂质过氧化产物已经被报道通过葡萄糖诱导的氧化应激形成。我们在糖尿病患者的红细胞膜磷脂中发现了9-羟基亚油酸（9-OH-C18:2）。我们假设9-OH-C18:2是在葡萄糖诱导的氧化应激过程中与亚油酸（C18:2）发生羟基自由基反应生成的，并证实合成的C18:2是由紫外线(UV)-C照射诱导生成的，紫外线-C光产生高活性的羟基自由基。C18:2被证实是9-OH-C18:2的前体。为了评估氧化对红细胞膜磷脂的损伤程度，我们开发了一种选择性离子监测气相色谱-质谱法测量C18:2和9-OH-C18:2。红细胞膜磷脂的甲醇分解。C18:2的相对峰高比。测定了健康人（n = 29, 3.1 +/- 1.9%）和糖尿病人（n = 27, 20.9 +/- 16.1%）红细胞磷脂提取物中9-OH-C18:2 （9-OH-C18:2/C18:2）的含量。证实9-OH-C18:2/C18:2在糖尿病患者中显著升高（P < 0.001）。红细胞9-OH-C18:2/C18:2的测定可用于评估糖尿病患者细胞膜磷脂的氧化损伤。(C) 1999 Elsevier Science B.V.版权所有
The increased production of oxygen-derived free radicals (OFR) and lipid peroxidation may contribute to vascular complications in diabetes. Some lipid peroxidation products have already been reported to be formed via glucose-induced oxidative stress. We have identified 9-hydroxy linoleic acid (9-OH-C18:2) in the red cell membrane phospholipid of diabetic subjects. We hypothesized that 9-OH-C18:2 would be formed in hydroxyl radical reactions to linoleic acid (C18:2) during glucose-induced oxidative stress, and confirmed that the formation of 9-OH-C18:2 was induced by ultraviolet (UV)-C irradiation to the synthetic C18:2, UV-C light generates highly reactive hydroxy radicals. C18:2 is confirmed to be the precursor of 9-OH-C18:2. To estimate the degree of oxidative damage to red cell membrane phospholipids, we developed a selective ion monitoring gas chromatography-mass spectrometric measurement for C18:2 and 9-OH-C18:2. following methanolysis of red cell membrane phospholipids. The relative peak height ratio of C18:2. to 9-OH-C18:2 (9-OH-C18:2/C18:2) was measured in phospholipid extracts of red cell membranes from healthy (n = 29, 3.1 +/- 1.9%) and diabetic (n = 27, 20.9 +/- 16.1%) subjects. It was confirmed that 9-OH-C18:2/C18:2 is significantly (P < 0.001) elevated in patients with diabetes. The measurement of 9-OH-C18:2/C18:2 in red cell membranes should be useful for assessing oxidative damage to membrane phospholipids in diabetes. (C) 1999 Elsevier Science B.V. All rights reserved.