A novel and sensitive method for the detection of enrofloxacin in food using time-resolved fluoroimmunoassay
A novel and sensitive method for the detection of enrofloxacin in food using time-resolved fluoroimmunoassay
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DOI：
10.3109/15376516.2012.757685
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发表时间：
2013-01
影响因子：
3.2
通讯作者：
Z. Bin;Z. Kai;Z. Jue;W. Ke;Zhao Lili;Jin Jian;H. Biao
中科院分区：
文献类型：
--
作者：
Z. Bin;Z. Kai;Z. Jue;W. Ke;Zhao Lili;Jin Jian;H. Biao
摘要建立了一种检测食品中恩诺沙星污染的时间分辨荧光免疫分析技术。利用ENR-卵清蛋白、抗ENR抗体和铕标记羊抗兔抗体，建立了间接竞争性ENR-TRFIA检测方法。方法灵敏度高，检出限为0.01 ng/mL。实验表明，该方法的灵敏度在鳗鱼、猪肉和鸡肉中为1 μg/kg，在蜂蜜中为1 μg/L。达到的检测范围为0.01-100 ng/mL，在检测范围内，ENR-TRFIA方法的批内和批间变异系数分别为2.4%和9.2%。用TRFIA和酶联免疫分析法对鳗鱼样品进行检测，结果吻合较好。该检测方法与食品中常见的其他喹诺酮类药物无交叉反应。研究表明，ENR-TRFIA是一种简便、灵敏、经济的大批量样品筛选方法，具有良好的应用前景。
Abstract A time-resolved fluoroimmunoassay (TRFIA) technique is developed to detect enrofloxacin (ENR) contamination in food. By using ENR-ovalbumin, anti-ENR antibodies and europium-labeled goat anti-rabbit antibodies, we establish an indirect and competitive method for ENR-TRFIA. The sensitivity of the method is high, with a detection limit of 0.01 ng/mL. The tests show that the technique’s sensitivity is 1 μg/kg in eel, pork and chicken, and 1 μg/L in honey. The detection range attained is 0.01–100 ng/mL and within the detection range the intra- and inter-batch coefficients of variation of the ENR-TRFIA method are 2.4% and 9.2%, respectively. The data obtained from eel samples by TRFIA and enzyme-linked immunoassay are in good agreement. The assay did not cross-react with other quinolones, which commonly exist in food. The study suggests that ENR-TRFIA is a simple, sensitive and economic method of screening large quantities of samples, and has good prospects for application.