Metabolic versatility of halotolerant and alkaliphilic strains of Halomonas isolated from alkaline black liquor.
Metabolic versatility of halotolerant and alkaliphilic strains of Halomonas isolated from alkaline black liquor.
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DOI：
10.1016/j.biortech.2010.03.108
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发表时间：
2010-09
影响因子：
11.4
通讯作者：
Chunyu Yang;Zhe Wang;Yang Li;Y. Niu;Miaofen Du;Xiaofei He;Cuiqing Ma;Hongzhi Tang;P. Xu
中科院分区：
文献类型：
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作者：
Chunyu Yang;Zhe Wang;Yang Li;Y. Niu;Miaofen Du;Xiaofei He;Cuiqing Ma;Hongzhi Tang;P. Xu
麦秆黑液是一种臭名昭著的纸浆厂废水，具有极高的 pH 值和污染负荷。两种耐盐和嗜碱细菌，命名为盐单胞菌属 (Halomonas sp.)。 19-A和Y2是从麦秆黑液中分离出来的，并且显示能够在pH 9.5和10% NaCl存在下使用愈创木酚、香草醛、二苯并-对二恶英、联苯和芴作为唯一碳源，并使用咔唑作为唯一碳源和氮源。这两个菌株产生羧甲基纤维素酶（CMCase）、木聚糖酶、脂肪酶、淀粉酶和支链淀粉酶。在 pH 5.0-11.0 和 NaCl 浓度 0-15% 时观察到 CMCase、木聚糖酶和淀粉酶的高活性。即使在极端 pH 和盐度条件下，这些盐单胞菌菌株的代谢多功能性使它们成为生物修复和工业过程的有希望的试剂。
Wheat straw black liquor is a notorious pulp mill wastewater with very high pH and pollution load. Two halotolerant and alkaliphilic bacteria, designated as Halomonas sp. 19-A and Y2, were isolated from wheat straw black liquor and shown to be able to use guaiacol, vanillin, dibenzo-p-dioxin, biphenyl and fluorene, as sole carbon and carbazole as sole carbon and nitrogen source at pH 9.5 and in the presence of 10% NaCl. The two strains produced carboxymethylcellulase (CMCase), xylanase, lipase, amylase, and pullulanase. High activities of CMCase, xylanase, and amylase were observed at pH 5.0–11.0 and NaCl concentrations of 0–15%. The metabolic versatility of these Halomonas strains even under extreme pH and salinity conditions makes them promising agents for bioremediation and industrial processes.