Circular RNA cSMARCA5 regulates the progression of cervical cancer by acting as a microRNA-432 sponge
Circular RNA cSMARCA5 regulates the progression of cervical cancer by acting as a microRNA-432 sponge
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DOI：
10.3892/mmr.2020.10910
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发表时间：
2020-03-01
影响因子：
3.4
通讯作者：
Li, Qian
中科院分区：
文献类型：
--
作者：
Huang, Ping;Qi, Bingli;Li, Qian
环状RNA（circRNA）已被证明参与癌症的发展。本研究的目的是研究circRNA SMARCA 5（cSMARCA 5）在人宫颈癌中的作用。在本研究中，cSMARCA 5表达在宫颈癌组织和细胞系中上调。此外，用表达靶向cSMARCA 5的小干扰RNA的病毒质粒转导的细胞的增殖速率下调。生物信息学分析预测microRNA（miR）-432靶向cSMARCA 5，并且miR-432能够通过结合表皮生长因子受体（EGFR）的3 '-非翻译区与EGFR相互作用。EGFR、ERK 1和ERK 2在宫颈癌组织中的表达水平升高。相关性分析显示cSMARCA 5与ERK 1和ERK 2水平呈正相关。总之，本研究结果表明，cSMARCA 5可能通过调节miR-432通过ERK信号通路在宫颈癌的进展中发挥重要作用。
Circular RNAs (circRNAs) have been shown to be involved in the development of cancer. The aim of the present study was to investigate the role of circRNA SMARCA5 (cSMARCA5) in human cervical cancer. In the present study, cSMARCA5 expression was upregulated in cervical cancer tissues and cell lines. Furthermore, the proliferation rate of cells transduced with viral plasmids expressing small interfering RNA targeting cSMARCA5 was downregulated. Bioinformatics analysis predicted that microRNA (miR)-432 targeted cSMARCA5, and miR-432 was able to interact with epidermal growth factor receptor (EGFR) by binding to its 3 '-untranslated region. The expression levels of EGFR, ERK1 and ERK2 were increased in cervical cancer tissues. Furthermore, correlation analysis revealed that cSMARCA5 levels were positively correlated with ERK1 and ERK2 levels. In conclusion, the present findings suggested that cSMARCA5 may play an important role in the progression of cervical cancer via the ERK signaling pathway by modulating miR-432.