IDENTIFICATION OF FIBROUS PROTEINS IN FETAL RAT EPIDERMIS BY ELECTROPHORETIC AND IMMUNOLOGICAL TECHNIQUES
IDENTIFICATION OF FIBROUS PROTEINS IN FETAL RAT EPIDERMIS BY ELECTROPHORETIC AND IMMUNOLOGICAL TECHNIQUES
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DOI：
10.1111/1523-1747.ep12482148
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发表时间：
1976-01-01
影响因子：
6.5
通讯作者：
HUANG, LY
中科院分区：
文献类型：
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作者：
DALE, BA;STERN, IB;HUANG, LY
从胎鼠皮肤提取物中鉴定出与新生大鼠角质层2种主要纤维蛋白相关的蛋白(2)。经十二烷基硫酸钠(SDS)-聚丙烯酰胺凝胶电泳和纯化纤维蛋白抗体免疫电泳检测，从妊娠第16 ~ 20天，这些蛋白的相对数量逐日增加。sds -聚丙烯酰胺凝胶电泳和免疫电泳的二维分析表明，这两种蛋白是16 -19天胎儿皮肤中唯一具有交叉反应的蛋白。在20d和21d检测到一些额外的低分子量成分。在双扩散分析中，19天胎儿提取物中的交叉反应蛋白与20天胎儿提取物中的交叉反应蛋白具有部分同质性，但抗原位点较少。20d蛋白与纯化的新生纤维蛋白表现出同源性反应。胎儿皮肤的免疫荧光研究支持在第16天发育时与新生儿纤维蛋白相关的交叉反应成分的存在。棘状细胞和颗粒状细胞的细胞质以及角膜透明素颗粒的荧光强度在18d和20d增加。角质层，第一次看到在第20天，是强烈的荧光。交叉反应蛋白的细胞定位和出现时间表明它们可能与张力丝有关。
Proteins (2) were identified in extracts of fetal rat skin which are related to the 2 major fibrous proteins of newborn rat stratum corneum. The relative amount of these proteins increased daily from the 16th-20th day (d) of gestation when judged by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and immunoelectrophoresis using antibody to the purified fibrous protein. Two-dimensional analysis by SDS-polyacrylamide gel electrophoresis and immunoelectrophoresis demonstrated that these 2 proteins are the only cross-reactive species in the fetal skin from 16d-19d development. Some additional lower molecular weight components were detected at 20d and 21d. In double-diffusion analysis, cross-reactive proteins in 19d fetal extracts showed partial identity but had fewer antigenic sites than proteins in 20d extracts. The 20d protein showed a reaction of identity with purified newborn fibrous protein. Immunofluorescence studies on fetal skin support the presence of cross-reacting components at 16d development related to the newborn fibrous protein. Intensity of fluorescence increases at 18d and 20d in the spinous and granular cell cytoplasm and in the keratohyaline granules. The stratum corneum, first seen at 20d, was intensely fluorescent. The cellular localization and time of appearance of the cross-reactive proteins suggest that they may be associated with tonofilaments.