Construction of a bioreactor to produce special breakdown products of phytate.
Construction of a bioreactor to produce special breakdown products of phytate.
复制标题
建造生物反应器来生产植酸盐的特殊分解产物。
DOI：
10.1016/0168-1656(96)01505-2
复制
发表时间：
1996
影响因子：
4.1
通讯作者：
U. Konietzny
中科院分区：
文献类型：
--
作者：
R. Greiner;U. Konietzny
将大肠杆菌植酸酶共价固定在NHS活化的Sepharose®High Performance上。植酸酶活性的pH依赖性不受固定化的影响，而对热处理的稳定性增强作为固定化的结果。与游离植酸酶相比，固定化植酸酶具有同样优异的底物特异性，但Km值增加。在填充床生物反应器中使用固定化植酸酶可获得低级肌醇磷酸酯的特殊异构体。生成的主要异构体经鉴定为I（1，2，3，4，5）P5、I（2，3，4，5）P4、I（2，4，5）P3和I（2，5）P2。
Escherichia coli phytase was covalently immobilized on NHS-activated Sepharose®High Performance. The pH dependence of the phytase activity was not influenced by immobilization, whereas stability against heat treatment was enhanced as a consequence of immobilization. Compared to the free phytase the immobilized enzyme exhibits the same excellent substrate specifity, but showed an increased Km-value. Using the immobilized phytase in a packed-bed bioreactor makes special isomers of the lower myo-inositol phosphate esters available. The major isomers formed were identified as I(1,2,3,4,5)P5, I(2,3,4,5)P4, I(2,4,5)P3and I(2,5)P2.