Evolutionarily conserved cytoprotection provided by Bax Inhibitor-1 homologs from animals, plants, and yeast
Evolutionarily conserved cytoprotection provided by Bax Inhibitor-1 homologs from animals, plants, and yeast
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DOI：
10.1016/j.gene.2003.09.011
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发表时间：
2003-12-24
期刊：
影响因子：
3.5
通讯作者：
Reed, JC
中科院分区：
文献类型：
--
作者：
Chae, HJ;Ke, N;Reed, JC
细胞程序性死亡（PCD）在动物和植物的发育和生理中起着重要作用，但目前还不清楚是否有类似的机制。Bax抑制剂-1（BI-1）是一种细胞内跨多膜蛋白和细胞死亡抑制剂，最初通过基于功能的筛选鉴定出能够抑制酵母中细胞死亡的哺乳动物cDNA，所述酵母工程化以异位表达促凋亡蛋白Bax。使用这种酵母试验，我们筛选了来自植物，番茄（番茄）和无脊椎动物果蝇（果蝇）的cDNA表达文库，鉴定了BI-1的密切同源物作为抑制因子。我们研究了BI-1的苍蝇和番茄同源物，以及在拟南芥、水稻和酿酒酵母中鉴定的BI-1同源物。当在酵母中表达时，BI-1的所有真核同源物都阻断了E2诱导的细胞死亡。真核生物BI-1同源物也部分拯救酵母细胞死亡诱导的氧化应激（H2 O2）和热休克。从BI-1同源物的C-末端结构域的缺失废除了它们在酵母中的细胞保护功能，表明这些蛋白质之间的保守的结构-功能关系。通过农杆菌渗入完整植物叶片表达番茄BI-1提供了对热激和冷激胁迫引起的损害的保护。总之，这些研究结果表明，BI-1同系物存在于多个真核生物物种，提供细胞保护，对不同的刺激，从而意味着BI-1调节进化保守的机制，植物和动物的应激抗性密切相关。(C)2003 Elsevier B. V.保留所有权利。
Programmed cell death (PCD) plays important roles in the development and physiology of both animals and plants, but it is unclear whether similar mechanisms are employed. Bax Inhibitor-1 (BI-1) is an intracellular multi-membrane-spanning protein and cell death inhibitor, originally identified by a function-based screen for mammalian cDNAs capable of suppressing cell death in yeast engineered to ectopically express the pro-apoptotic protein Bax. Using this yeast assay, we screened expression libraries for cDNAs from the plant, Lycopersicon esculentum (tomato), and the invertebrate animal Drosophila melanogaster (fruit fly), identifying close homologs of BI-1 as Bax-suppressors. We studied the fly and tomato homologs of BI-1, as well as BI-1 homologs identified in Arabidopsis thaliana, Oryza sativa (rice), and Saccharomyces cerevisiae (budding yeast). All eukaryotic homologs of BI-1 blocked Bax-induced cell death when expressed in yeast. Eukaryotic BI-1 homologs also partially rescued yeast from cell death induced by oxidative stress (H2O2) and heat shock. Deletion of a C-terminal domain from BI-1 homologs abrogated their cytoprotective function in yeast, demonstrating conserved structure-function relations among these proteins. Expression of tomato BI-1 by agroinfiltration of intact plant leaves provided protection from damage induced by heat-shock and cold-shock stress. Altogether, these findings indicate that BI-1 homologs exist in multiple eukaryotic species, providing cytoprotection against diverse stimuli, thus implying that BI-1 regulates evolutionary conserved mechanisms of stress resistance that are germane to both plants and animals. (C) 2003 Elsevier B.V. All rights reserved.