Evidence for microwave carcinogenesis in vitro.
Evidence for microwave carcinogenesis in vitro.
复制标题
微波体外致癌的证据。
DOI：
10.1093/carcin/6.6.859
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发表时间：
1985
期刊：
影响因子：
4.7
通讯作者：
G. H. Harrison
中科院分区：
文献类型：
--
作者：
E. Balcer;G. H. Harrison
采用体外恶性转化实验，研究了2.45 GHz微波辐射(MW)与苯并[a]芘(BP)或X射线联合对C3H/10T1/2小鼠胚胎成纤维细胞的致癌作用。实验还评估了无细胞毒性和非转化浓度的促癌剂12-O-十四酰佛波醇-13-乙酸酯(TPA)对微波和X射线诱导细胞转化的影响。实验是在低入射功率密度下进行的，对应的能量吸收速率为4.4W/kg。细胞在37.2℃+/-0.1℃处理，MW降低50%的培养效率，而TPA使其增加40%。在没有促癌剂的情况下，Mw对BP或X射线诱导的转化没有影响。与单独照射1.5GyX射线和4.5GyX射线的细胞相比，预先用微波和X射线照射的细胞经TPA处理后，转化频率分别增加了3.5倍和1.6倍。我们的结果表明，低水平的2.45 GHz毫瓦辐射可以诱发潜在的转化损伤，这种损伤可以通过肿瘤促进剂的作用来揭示。
We investigated the carcinogenic activity of 2.45 GHz microwave radiation (MW) combined with benzo[a]pyrene (BP) or X-rays, using an in vitro assay for malignant transformation in C3H/10T1/2 mouse-embryo fibroblasts. Additional experiments were performed to assess the effect of a non-cytotoxic and non-transforming concentration of the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) on transformation induction in cells treated with MW and X-rays. Experiments were performed at low incident power density, corresponding to an energy absorption rate of 4.4 W/kg. Cells were treated at 37.2+/-0.1 degree C. MW reduced the plating efficiency of 50%, while TPA increased it by 40%. MW had no effect on transformation induced by BP or X-rays in the absence of tumor promoter. TPA treatment of cells previously irradiated with MW and X-rays yielded a statistically significant 3.5- or 1.6-fold increase in transformation when compared with the transformation frequency of cells previously irradiated with X-rays alone at 1.5 and 4.5 Gy, respectively. Our results suggest that low-level 2.45 GHz MW radiation can induce latent transformation damage which can then be revealed by the action of tumor promoters.