Evaluation of PCR primers to screen for Streptococcus pneumoniae isolates and beta-lactam resistance, and to detect common macrolide resistance determinants.
Evaluation of PCR primers to screen for Streptococcus pneumoniae isolates and beta-lactam resistance, and to detect common macrolide resistance determinants.
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评估 PCR 引物筛选肺炎链球菌分离株和 β-内酰胺耐药性，并检测常见的大环内酯类耐药决定因素。
DOI：
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发表时间：
2001
影响因子：
5.2
通讯作者：
P. Appelbaum
中科院分区：
文献类型：
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作者：
K. Nagai;Y. Shibasaki;K. Hasegawa;T. Davies;M. R. Jacobs;K. Ubukata;P. Appelbaum
来自不同国家（主要来自美国）的肺炎球菌分离株（n = 148）通过PCR引物集进行检测。44株青霉素G敏感株（MIC ≤ 0.06 mg/L）中，38株（86.4%）pbps无变化，6株（13.6%）pbps有1 ~ 2个变化。47株青霉素G耐药菌株（MIC ≥ 2 mg/L）中，41株（87.2%）pbp全部改变，6株（12.8%）pbp 1a + 2x改变。在青霉素G中间分离株中观察到pbp改变的各种组合。MIC能清楚地反映大环内酯类药物耐药基因mef（A）和lytA（B）在分离株中的流行情况，所有分离株均为lytA阳性。该引物可用于肺炎链球菌和β-内酰胺类抗生素耐药的筛查，以及常见大环内酯类抗生素耐药决定簇的检测。
Pneumococcal isolates (n = 148) from various countries (mostly from the USA) were tested by a primer set for PCR. Thirty-eight (86.4%) of the 44 penicillin G-susceptible isolates (MIC < or = 0.06 mg/L) had unaltered pbps, while six isolates (13.6%) had either one or two alterations in pbps. Of 47 penicillin G-resistant strains (MIC > or = 2 mg/L), 41 isolates (87.2%) had all three pbps altered, six isolates (12.8%) had altered pbp1a + 2x. Various combinations of altered pbp were seen in penicillin G-intermediate isolates. Prevalence of macrolide resistance genes mef(A) and erm(B) in isolates was clearly reflected by their MICs. All isolates were positive for lytA. The primers were useful for screening for Streptococcus pneumoniae and beta-lactam resistance, and for detection of common macrolide resistance determinants.