Differences in the regulation of CD4 and CD8 T-cell clones during immune responses
Differences in the regulation of CD4 and CD8 T-cell clones during immune responses
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DOI：
10.1098/rstb.2000.0580
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发表时间：
2000-03-29
影响因子：
6.3
通讯作者：
Maini, MK
中科院分区：
文献类型：
--
作者：
Beverley, PCL;Maini, MK
免疫应答的功能单位是淋巴细胞克隆。对体内淋巴细胞寿命的分析表明，CD4和CD8淋巴细胞的总体周转没有太大差异。最近，分子方法已经发展起来，可以对体内对抗原反应的t细胞克隆进行全局分析。我们使用了一种敏感的、改良的异双工分析来跟踪急性感染性单核细胞增多症（AIM）中对eb病毒有反应的t细胞克隆。引人注目的是，在新分离的AIM血液中检测到的许多大克隆都在CD8部分中被发现。对可溶性回忆抗原破伤风圆环抗原应答的CD4克隆群体只有在体外再刺激后才能检测到。这些数据暗示CD4反应可能比CD8细胞更具有多克隆性，并且CD4克隆的大小受到更严格的调节。一些分子机制可能有助于此。端粒酶的上调允许CD8细胞在不耗尽增殖能力的情况下大量扩增。
The functional units of immune response are lymphocyte clones. Analysis of lymphocyte life span in vivo shows that the overall turnover of CD4 and CD8 lymphocytes does not differ greatly. Recently, molecular methods have been developed which allow a global analysis of T-cell clones responding to an antigen in vivo. We have used a sensitive, modified heteroduplex analysis to follow T-cell clones responding to Epstein-Barr virus in acute infectious mononucleosis (AIM). Strikingly, all the many large clones detected in freshly isolated AIM blood were found within the CD8 fraction. CD4 clonal populations responding to the soluble recall antigen tetanus toroid could only be detected after in vitro re-stimulation. These data imply that CD4 responses may be more polyclonal than those of CD8 cells and that the size of CD4 clones is more tightly regulated. Several molecular mechanisms may contribute to this. Up-regulation of telomerase allows very large expansions of CD8 cells to occur without exhaustion of proliferative capacity.