Biologically active pituitary hormones in the rat brain amygdaloid nucleus.
Biologically active pituitary hormones in the rat brain amygdaloid nucleus.
复制标题
大鼠大脑杏仁核中具有生物活性的垂体激素。
DOI：
10.1126/science.203034
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发表时间：
1978
期刊：
影响因子：
56.9
通讯作者：
A. Lawrence
中科院分区：
文献类型：
--
作者：
S. T. Pacold;L. Kirsteins;S. Hojvat;A. Lawrence
当人们试图确定存在于人类脑脊液中的生长激素释放因子的来源时，人们发现，在组织培养中生长的大鼠杏仁核细胞产生的物质在免疫和层析上与在脑垂体中发现的生长激素相同。免疫过氧化物酶染色显示过氧化物酶-抗生长激素复合体抗体在杏仁核细胞内密集聚集。大量的生长激素和促肾上腺皮质激素可以从这个边缘结构中提取出来。采用生物测定法对去垂体大鼠的生长激素免疫当量提取物进行测定。杏仁体提取物对骨骺软骨生长的促进作用与垂体前叶提取物的刺激作用相当。杏仁核提取物对肾上腺和性腺的大小和重量以及对甲状腺滤泡上皮生长的促进作用也与垂体提取物相当。
While an attempt was being made to identify the source of the growth hormone releasing factor present in cerebral spinal fluid of man, it was discovered that cells of the rat amygdaloid nucleus, grown in tissue culture, produce a material that is immunologically and chromatographically identical to growth hormone found in the pituitary. Immunoperoxidase staining revealed dense accumulation of the peroxidase-antibody to growth hormone complex in amygdala cells. Significant amounts of growth hormone and adrenocorticotropin could be extracted from this limbic structure. Extracts containing immunoequivalent amounts of growth hormone were measured by bioassay in hypophysectomized rats. Stimulation of the growth of epiphyseal cartilage by extracts of the amygdala was comparable to the stimulation by extracts of anterior pituitary glands. The stimulatory effect of amygdala extracts on adrenal and gonadal size and weight and on growth of thyroid follicular epithelium was also comparable to that of pituitary extracts.