Electrospun polystyrene/oxidized carbon nanotubes film as both sorbent for thin film microextraction and matrix for matrix-assisted laser desorption/ionization time-of-flight Mass spectrometry
Electrospun polystyrene/oxidized carbon nanotubes film as both sorbent for thin film microextraction and matrix for matrix-assisted laser desorption/ionization time-of-flight Mass spectrometry
复制标题
电纺聚苯乙烯/氧化碳纳米管薄膜既作为薄膜微萃取的吸附剂，又作为基质辅助激光解吸/电离飞行时间质谱的基质
DOI：
10.1016/j.chroma.2014.05.045
复制
发表时间：
2014-07-18
影响因子：
4.1
通讯作者：
Feng, Yu-Qi
中科院分区：
文献类型：
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作者：
He, Xiao-Mei;Zhu, Gang-Tian;Feng, Yu-Qi
本研究首次制备了聚苯乙烯/氧化碳纳米管（PS/OCNTs）薄膜，并将其用作薄膜微萃取（TFME）的吸附剂和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）的基质。通过静电纺丝获得了尺寸均匀的PS/OCNTs薄膜，OCNTs均匀牢固地固定在PS中。利用所制备的 PS/OCNTs 薄膜开发了一种新型 TFME 装置，以富集水中的苯并[a]芘 (BaP)，以及尿样中的 BaP 和 1-羟基芘 (1-OHP)。然后将PS/OCNTs膜上提取的分析物直接应用于以PS/OCNTs膜作为MALDI基质的MALDI-MS分析。我们的结果表明，PS/OCNT 薄膜是一种良好的分析物 TFME 吸附剂，也是使用 MALDI-TOF-MS 灵敏测定 BaP 和 1-OHP 的优异基质。采用PS/OCNT作为MALDI的基质可以有效避免通常由于基质层上吸附的分析物的不均匀分布而导致的信号强度的较大变化，从而显着提高点与点之间的重现性。在薄膜中引入PS可以防止OCNT从MALDI板中飞出损坏设备。此外，与 OCNT 基质相比，PS/OCNT 薄膜还大大延长了目标分析物离子信号的持续时间。该方法进一步成功用于定量测定环境水中的BaP和尿样中的1-OHP。结果表明，BaP和1-OHP分别在50 pg mL(-1)和500 pg mL(-1)浓度下即可轻松检出，表明该方法检测灵敏度较高。对于BaP分析，线性范围为0.1-20 ng mL(-1)，相关系数为0.9970，RSD回收率为81.3%至123.4%。
In the current study, polystyrene/oxidized carbon nanotubes (PS/OCNTs) film was prepared and applied as both an adsorbent of thin film microextraction (TFME) and matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for the first time. The uniform size of PS/OCNTs film with OCNTs evenly and firmly immobilized in PS was obtained by electrospinning. And a novel TFME device was developed using the prepared PS/OCNTs film to enrich benzo[a]pyrene (BaP) from water, and also BaP and 1-hydroxypyrene (1-OHP) from urine sample. Then the extracted analytes on the PS/OCNTs film were directly applied to MALDI-MS analysis with PS/OCNTs film as the MALDI matrix. Our results show that PS/OCNTs film is a good TFME adsorbent toward the analytes and an excellent matrix for the sensitive determination of BaP and 1-OHP using MALDI-TOF-MS. The employment of PS/OCNTs as the matrix for MALDI can effectively avoid the large variation of signal intensity normally resulting from heterogeneous distribution of the adsorbed analyte on matrix layer, which therefore significantly improve spot-to-spot reproducibility. The introduction of PS in the film can prevent OCNTs from flying out of MALDI plate to damage the equipment. In addition, PS/OCNTs film also largely extended the duration of ion signal of target analyte compared to OCNTs matrix. The developed method was further successfully used to quantitatively determine BaP in environmental water and 1-OHP in urine samples. The results show that BaP and 1-OHP could be easily detected at concentrations of 50 pg mL(-1) and 500 pg mL(-1), respectively, indicating the high detection sensitivity of this method. For BaP analysis, the linear range was 0.1-20 ng mL(-1) with a correlation coefficient of 0.9970 and the recoveries were in the range of 81.3 to 123.4% with the RSD