Distinct tomato yellow leaf curl Chuxiong virus isolated from whiteflies and plants in China and its symptom determinant and suppressor of post-transcriptional gene silencing
Distinct tomato yellow leaf curl Chuxiong virus isolated from whiteflies and plants in China and its symptom determinant and suppressor of post-transcriptional gene silencing
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DOI：
10.1016/j.virol.2024.110040
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发表时间：
2024-03-11
期刊：
影响因子：
3.7
通讯作者：
Ding，Ming
中科院分区：
文献类型：
--
作者：
Xie，Yan;Liu，Xianan;Ding，Ming
从中国烟粉虱（Bemisia tabaci）和番茄、甘薯中分离出的 Begomovirus 是一种独特 Begomovirus 的代表，建议将其命名为番茄黄曲叶楚雄病毒（TYLCCxV）。基因组鉴定和序列比对结果表明，TYLCCxV与巴豆黄脉花叶病毒（CroYVMV）的完整核苷酸序列一致性最高（88.3%），可能来源于西尼德雷拉卷叶病毒（SyLCV）和南瓜曲叶云南病毒（SLCuYV）的重组。农杆菌介导的接种表明，TYLCCxV 对一系列植物物种具有高度传染性，可导致番茄植物出现叶片向上卷曲、叶片皱缩、失绿、扭曲和矮化症状。 Southern blot结果表明TYLCCxV能够有效复制两个异源β卫星。在本塞姆氏烟草上接种 PVX::C4 会诱导叶片向上卷曲和茎伸长症状，表明 TYLCCxV C4 作为症状决定因素发挥作用。 TYLCCxV V2 是一种重要的毒力因子，可在 co-agroinfilteratedN 中诱导下卷叶症状、引发全身性坏死并抑制局部和全身 GFP 沉默。 Benthamiana 和转基因 16c 植物。考虑到多功能毒力蛋白V2和C4，讨论了TYLCCxV在中国引起番茄毁灭性流行的可能性。
A begomovirus isolated from whiteflies (Bemisia tabaci) and tomato, sweet potato in China was found to be representative of a distinct begomovirus species, for which the name tomato yellow leaf curl Chuxiong virus (TYLCCxV) is proposed. The results of genomic identification and sequence comparison showed that TYLCCxV shares the highest complete nucleotide sequence identity (88.3%) with croton yellow vein mosaic virus (CroYVMV), and may have originated from the recombination between synedrella leaf curl virus (SyLCV) and squash leaf curl Yunnan virus (SLCuYV). Agrobacterium-mediated inoculation showed that TYLCCxV is highly infectious for a range of plant species, producing upward leaf curling, leaf crumpling, chlorosis, distortion, and stunt symptoms inSolanum lycopersicumplants. The results of Southern blot indicated that TYLCCxV is capable of efficiently replicating two heterologous betasatellites. The inoculation of PVX::C4 onNicotiana benthamianainduced upward leaf curling and stem elongation symptoms, suggesting that TYLCCxV C4 functions as a symptom determinant. TYLCCxV V2 is an important virulence factor that induces downward leaf curling symptoms, elicits systemic necrosis, and suppresses local and systemic GFP silencing in co-agroinfiltratedN. benthamianaand transgenic 16c plants. Considering the multifunctional virulence proteins V2 and C4, the possibility of TYLCCxV causing devastating epidemics on tomato in China is discussed.