Nanofilament silicon for matrix-free laser desorption/ionization mass spectrometry.
Nanofilament silicon for matrix-free laser desorption/ionization mass spectrometry.
复制标题
用于无基质激光解吸/电离质谱分析的纳米丝硅。
DOI：
10.1007/978-1-61779-319-6_14
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Devoe,DonL
中科院分区：
文献类型：
--
作者：
Tsao,Chia-Wen;Devoe,DonL
质谱法（MS）提供了定量信息，以准确的质量鉴定未知分子，并已成为一个强大的和广泛使用的生物分析技术。在这一章中，我们描述了高灵敏度无基质激光解吸/电离质谱（LDI-MS）的目标基板称为纳米丝硅（nSi）的制造和MS分析步骤。与其他纳米结构的多孔硅表面不同，nSi具有开孔形态，具有相关的益处，包括将样品有效地输送到纳米孔中以及从表面有效地去除溶剂。用于LDI-MS分析的nSi靶的效用主要在于小分子的分析，其通常小于5，000 Da，检测灵敏度在阿托摩尔范围内。
Mass spectrometry (MS) provides quantitative information toward accurate mass identification of unknown molecules and has become a powerful and widely used technique for bioanalysis. In this chapter, we describe the fabrication and MS analysis steps for a high sensitivity matrix-free laser desorption/ionization mass spectrometry (LDI-MS) target substrate termed nanofilament silicon (nSi). Unlike other nanostructured porous silicon surfaces, nSi possesses an open pore morphology with associated benefits including efficient transport of sample into the nanopores and effective removal of solvent from the surface. The utility of nSi targets for LDI-MS analysis lies primarily in the analysis of small molecules, which are typically less than 5,000 Da with a detection sensitivity in the attomole range.