Mechanisms of Selective Transport through the Nuclear Pore Complex
Mechanisms of Selective Transport through the Nuclear Pore Complex
复制标题
通过核孔复合体的选择性运输机制
DOI：
10.1016/j.bpj.2017.11.2375
复制
发表时间：
2018
影响因子：
3.4
通讯作者：
Hough, Loren
中科院分区：
文献类型：
--
作者：
Maguire, Laura;Stefferson, Michael;Betterton, Meredith;Hough, Loren
很少有细胞过程需要像通过核膜运输这样复杂的主动控制。核孔复合体(NPC)促进了所有的运输，阻止了大多数大分子穿过包膜，同时允许运输因子(TF)及其货物通过。虽然运输的基本生化相互作用已经被很好地理解，但详细的机制仍然是一个重要的争论话题。我们用反应扩散方程来模拟输运。结果表明：(1)无序FG-NUP的柔性性质和(2)FG-NUP-Tf相互作用的瞬变、多价性质共同具有足够的选择性。我们的模型对选择性机制做出了可直接检验的预测。
Few cellular processes require such intricate active control as transport through the nuclear envelope. The nuclear pore complex (NPC) facilitates all transport, preventing most macromolecules from crossing the envelope while allowing the passage of transport factors (TFs) and their cargo. While the basic biochemical interactions of transport are well-understood, the detailed mechanism remains a topic of significant debate. We model transport using reaction-diffusion equations. The results suggest that (1) the flexible nature of the disordered FG nups and (2) the transient, multivalent nature of FG nup-TF interactions are together sufficient for selectivity. Our model makes directly-testable predictions of the mechanisms of selectivity.