Sugar Chips immobilized with synthetic sulfated disaccharides of heparin/heparan sulfate partial structure.
Sugar Chips immobilized with synthetic sulfated disaccharides of heparin/heparan sulfate partial structure.
复制标题
糖片固定有肝素/硫酸乙酰肝素部分结构的合成硫酸化二糖。
DOI：
10.1016/j.bmcl.2008.01.069
复制
发表时间：
2008
影响因子：
2.7
通讯作者：
Suda,Yasuo
中科院分区：
文献类型：
--
作者：
Wakao,Masahiro;Saito,Akihiro;Ohishi,Koh;Kishimoto,Yuko;Nishimura,Tomoaki;Sobel,Michael;Suda,Yasuo
碳水化合物芯片技术在碳水化合物-蛋白质相互作用的高通量评估方面具有巨大潜力。在此，我们报道了具有肝素和硫酸乙酰肝素部分二糖结构的新型硫酸化寡糖的合成，将其固定在金涂层芯片上以制备阵列型糖芯片，并通过表面等离子共振（SPR）成像技术评估蛋白质的结合能力。由葡萄糖胺和糖醛酸部分有效合成硫酸化寡糖。然后使用先前报道的方法将合成的硫酸化寡糖轻松固定在镀金芯片上。该分析方法的有效性在芯片与肝素结合蛋白、纤连蛋白和重组人血管性血友病因子 A1 结构域 (rh-vWf-A1) 之间的结合实验中得到证实，其中鉴定了负责结合的肝素或硫酸乙酰肝素的特定部分结构。
Carbohydrate chip technology has a great potential for the high-throughput evaluation of carbohydrate–protein interactions. Herein, we report syntheses of novel sulfated oligosaccharides possessing heparin and heparan sulfate partial disaccharide structures, their immobilization on gold-coated chips to prepare array-type Sugar Chips, and evaluation of binding potencies of proteins by surface plasmon resonance (SPR) imaging technology. Sulfated oligosaccharides were efficiently synthesized from glucosamine and uronic acid moieties. Synthesized sulfated oligosaccharides were then easily immobilized on gold-coated chips using previously reported methods. The effectiveness of this analytical method was confirmed in binding experiments between the chips and heparin binding proteins, fibronectin and recombinant human von Willebrand factor A1 domain (rh-vWf-A1), where specific partial structures of heparin or heparan sulfate responsible for binding were identified.