FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells
FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells
复制标题
FEN1抑制剂增加宫颈癌细胞放疗的敏感性
DOI：
10.1002/cam4.2615
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发表时间：
2019-12-01
期刊：
影响因子：
4
通讯作者：
Liu, Song-Bai
中科院分区：
文献类型：
--
作者：
Li, Jin-Li;Wang, Jian-Ping;Liu, Song-Bai
Background: Cervical cancer is one of the most common causes of cancer-associated mortality among affected women in the world. At present, treatment with weekly cisplatin plus ionizing radiation (IR) therapy is the standard regimen for cervical cancer, especially for locally advanced cervical cancer. The purpose of this study is to determine whether FEN1 inhibitors could enhance the therapeutic effect of IR therapy.Methods: Western blot was applied to determine the expression of FEN1- and apoptosis-related proteins. Cell growth inhibition assay and colony formation assay were used to determine the effects of FEN1 inhibitor and IR exposure for Hela cells in vitro. CRISPR technology was used to knockdown FEN1 expression level of 293T cells, and tumor xenograft in nude mice was employed to determine the effects of FEN1 inhibitor and IR exposure on tumor growth in vivo.Results: Our data revealed that FEN1 is overexpressed in HeLa cell and can be upregulated further by IR. We also demonstrated that FEN1 inhibitor enhances IR sensitivity of cervical cancer in vitro and in vivo.Conclusion: FEN1 inhibitor SC13 could sensitize radiotherapy of cervical cancer cell.
