Origin and phenotypic features of hyperplastic epithelial cells in collapsing glomerulopathy
Origin and phenotypic features of hyperplastic epithelial cells in collapsing glomerulopathy
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DOI：
10.1016/s0272-6386(98)70070-8
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发表时间：
1998-12-01
影响因子：
13.2
通讯作者：
Watanabe, T
中科院分区：
文献类型：
--
作者：
Nagata, M;Hattori, M;Watanabe, T
崩解性肾小球病变(CG)的典型病理特征是肾小球内明显的细胞增生和肥大。本研究调查了慢性萎缩性胃炎增生性上皮细胞的表型变化，以确定其来源。对2例CG患者的肾活检标本进行免疫组织化学染色，检测足细胞(PHM-5)、壁上皮细胞(PECS；细胞角蛋白)和细胞增殖(Ki-67)。在萎缩性肾小球中，增生和肥大的上皮细胞常连接到PECS和塌陷的肾小球基底膜(GBM)，这些上皮细胞更多地是Ki-67阳性和表达细胞角蛋白，而PHM-5几乎都是阴性的。连续切片分析显示，少数增生的上皮细胞同时表达PHM-B和细胞角蛋白，提示足细胞和PECS之间的表型转换，而且细胞角蛋白阳性的细胞与硬化的肾小球节段有关。因此，我们认为大多数增生性和肥厚性上皮细胞起源于PEC，这些上皮细胞特征可能参与了CG特征性的绒毛塌陷和肾小球硬化的发生发展过程。(C)1998年，由国家肾脏基金会公司提供。
The characteristic pathological feature of collapsing glomerulopathy (CG) is marked cell hyperplasia and hypertrophy within the glomeruli. The present study investigated the phenotypic alteration of hyperplastic epithelial cells in CG to determine their origin. Renal biopsy specimens from two patients with CG were analyzed by immunohistochemical staining, using markers for podocytes (PHM-5), parietal epithelial cells (PECs; cytokeratin), and cell proliferation (Ki-67), In collapsed glomeruli, hyperplastic and hypertrophic epithelial cells were frequently connected to PECs and collapsed glomerular basement membranes (GBMs), These epithelial cells were more often Ki-67 positive and expressed cytokeratin, whereas PHM-5 was almost invariably negative, Serial section analysis showed that a small number of hyperplastic epithelial cells expressed both PHM-B and cytokeratin, suggesting phenotypic conversion between podocytes and PECs, Moreover, cytokeratin-positive cells were associated with the sclerotic glomerular segments. Thus, we suggest that the majority of hyperplastic and hypertrophic epithelial cells in CG are of PEC origin, These epithelial features may participate in the development of characteristic tuft collapse and glomerulosclerosis in CG. (C) 1998 by the National Kidney Foundation, Inc.