Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System.
Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System.
复制标题
使用杆状病毒表达载体系统在大规模搅拌生物反应器中生产重组蛋白。
DOI：
10.1007/978-1-4939-3043-2_11
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发表时间：
2016
影响因子：
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通讯作者：
A. Kamen
中科院分区：
文献类型：
--
作者：
Christine M Thompson;Johnny Montes;M. Aucoin;A. Kamen
本章讨论在大型搅拌生物反应器中使用杆状病毒表达载体系统（BEVS）生产重组蛋白。介绍了批量过程关键阶段的详细方法，包括宿主细胞生长、病毒储备扩增和定量、生物反应器准备和操作、感染过程、最终收获以及用于回收产物的初级分离步骤。此外，还简要讨论了涉及先进在线监测和生物反应器控制的方法，这些方法对整个过程的成功具有重大影响。
The production of recombinant proteins using the baculovirus expression vector system (BEVS) in large-scale agitated bioreactors is discussed in this chapter. Detailed methods of the key stages of a batch process, including host cell growth, virus stock amplification and quantification, bioreactor preparation and operation, the infection process, final harvesting, and primary separation steps for recovery of the product are presented. Furthermore, methods involved with advanced on-line monitoring and bioreactor control, which have a significant impact on the overall process success, are briefly discussed.