Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae
Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae
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DOI：
10.1073/pnas.0712221105
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发表时间：
2008-02-19
影响因子：
11.1
通讯作者：
Wakabayashi, Keiji
中科院分区：
文献类型：
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作者：
Matsumoto, Yasuko;Nakano, Tsuyoshi;Wakabayashi, Keiji
菜粉蝶（Pieris raisicae）和菜粉蝶（Pieris brassicae）含有针对癌细胞系的强细胞毒性蛋白，命名为粉蝶素-1和-2。这些蛋白质表现出DNA ADP-核糖基化活性。为了确定具有细胞毒性和DNA ADP-核糖基化活性的物质在其他物种中的分布，对粉蝶科20种粉蝶的粗提物在HeLa细胞中的细胞毒性和DNA ADP-核糖基化活性进行了检测。在13个物种的提取物中检测到这两种活性：粉蝶亚族（粉蝶、粉粉蝶、菜粉蝶、粉蝶、芸苔粉蝶、Pontia daplidice和Talbotia naganum）、Aporiina（Aporia pastea、Aporia pasta、Aporia hippia和Delias pasithoe）和Appiadina（Appias nero和Appias paulina）。所有这些提取物都含有被抗粉蝶素-1抗体识别的物质，其分子量约为100 kDa。此外，含有NAD-结合位点，保守的ADP-核糖基转移酶，从基因组DNA中扩增的序列从13种蝴蝶的细胞毒性和DNA ADP-核糖基化活性的PCR。七个物种的提取物，Appias lyncida，Leptosia nina，Anthocharis scolymus，Eurema hecabe，Catopsilia pomona，Catopsilia scylla和Colias erate，既没有细胞毒性，也没有DNA ADP-核糖基化活性，不含抗pierisin-1抗体识别的物质。从这七个物种的基因组DNA中没有扩增出含有NAD结合位点的序列。因此，粉蝶素样蛋白，显示细胞毒性和DNA ADP-核糖基化活性，建议存在于提取物中的蝴蝶不仅在亚族粉蝶，但也在亚族Aporiina和Appiadina。这些发现为理解蝴蝶中DNA ADP-核糖基化活性的性质提供了见解。
Cabbage butterflies, Pieris rapae and Pieris brassicae, contain strong cytotoxic proteins, designated as pierisin-1 and -2, against cancer cell lines. These proteins exhibit DNA ADP-ribosylating activity. To determine the distribution of substances with cytotoxicity and DNA ADP-ribosylating activity among other species, crude extracts from 20 species of the family Pieridae were examined for cytotoxicity in HeLa cells and DNA ADP-ribosylating activity. Both activities were detected in extracts from 13 species: subtribes Pierina (Pieris rapae, Pieris canidia, Pieris napi, Pieris melete, Pieris brassicae, Pontia daplidice, and Talbotia naganum), Aporiina (Aporia gigantea, Aporia crataegi, Aporia hippia, and Delias pasithoe), and Appiadina (Appias nero and Appias paulina). All of these extracts contained substances recognized by anti-pierisin-1 antibodies, with a molecular mass of approximate to 100 kDa established earlier for pierisin-1. Moreover, sequences containing NAD-binding sites, conserved in ADP-ribosyltransferases, were amplified from genomic DNA from 13 species of butterflies with cytotoxicity and DNA ADP-ribosylating activity by PCR. Extracts from seven species, Appias lyncida, Leptosia nina, Anthocharis scolymus, Eurema hecabe, Catopsilia pomona, Catopsilia scylla, and Colias erate, showed neither cytotoxicity nor DNA ADP-ribosylating activity, and did not contain substances recognized by anti-pierisin-1 antibodies. Sequences containing NAD-binding sites were not amplified from genomic DNA from these seven species. Thus, pierisin-like proteins, showing cytotoxicity and DNA ADP-ribosylating activity, are suggested to be present in the extracts from butterflies not only among the subtribe Pierina, but also among the subtribes Aporiina and Appiadina. These findings offer insight to understanding the nature of DNA ADP-ribosylating activity in the butterfly.