Origins of Cell-Type-Specific Olfactory Processing in the Drosophila Mushroom Body Circuit
Origins of Cell-Type-Specific Olfactory Processing in the Drosophila Mushroom Body Circuit
复制标题
DOI：
10.1016/j.neuron.2017.06.039
复制
发表时间：
2017-07-19
期刊：
影响因子：
16.2
通讯作者：
Kazama, Hokto
中科院分区：
文献类型：
--
作者：
Inada, Kengo;Tsuchimoto, Yoshiko;Kazama, Hokto
细胞类型特定的生理特性如何影响电路中的神经元功能仍然知之甚少。我们在果蝇蘑菇体(MB)中解决了这个问题，这是一种高级嗅觉回路，触角叶中属于不同小球的神经元将兴奋反馈给三种类型的内在神经元，即α/β、α‘/β’和伽马凯尼恩细胞(KCs)。双光子光遗传学和细胞内记录显示，虽然所有KC的肾小球输入增加相似，但在α‘/β’KC中最容易产生尖峰。这种类型的细胞也最有能力招募由前配对外侧神经元反馈的GABA能抑制，根据刺激强度的不同，外侧神经元对气味的反应可以是局部的，也可以是整个脑叶的。值得注意的是，正如从这些生理特性预测的那样，阿尔法/贝塔‘KCs具有最高的气味检测速度、灵敏度和辨别能力。这种增强的辨别能力需要适当的GABA能抑制。这些结果将MB回路中特定细胞类型的机制和功能联系在一起。
How cell-type-specific physiological properties shape neuronal functions in a circuit remains poorly understood. We addressed this issue in the Drosophila mushroom body (MB), a higher olfactory circuit, where neurons belonging to distinct glomeruli in the antennal lobe feed excitation to three types of intrinsic neurons, alpha/beta, alpha'/beta', and gamma Kenyon cells (KCs). Two-photon optogenetics and intracellular recording revealed that whereas glomerular inputs add similarly in all KCs, spikes were generated most readily in alpha'/beta' KCs. This cell type was also the most competent in recruiting GABAergic inhibition fed back by anterior paired lateral neuron, which responded to odors either locally within a lobe or globally across all lobes depending on the strength of stimuli. Notably, as predicted from these physiological properties, alpha'/beta' KCs had the highest odor detection speed, sensitivity, and discriminability. This enhanced discrimination required proper GABAergic inhibition. These results link cell-type-specific mechanisms and functions in the MB circuit.