Feline leukemia virus envelope gp70 of subgroups B and C defined by monoclonal antibodies with cytotoxic and neutralizing functions.
Feline leukemia virus envelope gp70 of subgroups B and C defined by monoclonal antibodies with cytotoxic and neutralizing functions.
复制标题
猫白血病病毒包膜有 B 和 C 亚型的 gp70，由具有细胞毒性和中和功能的单克隆抗体定义。
作者：
C. K. Grant;B. J. Ernisse;O. Jarrett;F. Jones
本文报道了9株抗猫白血病病毒（FeLV）包膜蛋白的鼠源单克隆抗体（MAb）。8个MAb针对gp 70的相同分子种类的表位，另一个MAb针对p15 E部分。六个gp 70表位是离散的;两个紧密相关或重叠。4种抗gp 70 MAb（2种IgG 2A亚类和2种IgG 2B亚类）与猫或兔补体一起对FeLV生产型淋巴瘤细胞具有直接细胞毒性（C）。另一种单克隆抗体（IgG 2B），这是不是细胞毒性单独，特异性和协同增加两个IgG 2A单克隆抗体的细胞毒性作用。细胞毒性抗gp 70 MA B b也具有病毒中和能力;一种MA B b识别所有FeLV亚组（A、B和C）共有的决定簇，其他MA b识别亚组A上不存在但B和C亚组共有的gp 70表位。采用单克隆抗体结合的竞争性抑制来绘制表位的空间分布，并且结果符合形状为不完整环的分子。根据该模型，涉及细胞毒性和病毒中和抗体功能的表位密切相关;涉及的区域大约位于分子的中心，并且它包含在来自不同猫淋巴瘤细胞系的FeLV个体分离株中表达的表位。
Nine murine monoclonal antibodies (MAb) to the envelope proteins of feline leukemia virus (FeLV) are described. Eight MAb are directed to epitopes of the same molecular species of gp70 and the other MAb is directed to the p15E moiety. Six of the gp70 epitopes are discrete; two are closely associated or overlapping. Four anti-gp70 MAb (2 of IgG2A and 2 of IgG2B subclasses) were directly cytotoxic for FeLV-producer lymphoma cells with cat or with rabbit complement (C). Another MAb (IgG2B), which was not cytotoxic alone, specifically and synergistically increased the cytotoxic effects of both IgG2A MAb. Cytotoxic anti-gp70 MAb also had virus-neutralizing capacity; one MAb recognized a determinant common to all FeLV subgroups (A, B, and C), the others recognized gp70 epitopes not present on subgroup A but common to both B and C subgroups. Competitive inhibition of MAb binding was employed to map spatial distributions of the epitopes, and the results fitted a molecule shaped as an incomplete loop. According to the model, epitopes involved with cytotoxic and virus neutralizing antibody functions were closely associated; the region involved is approximately in the center of the molecule, and it contains epitopes that are variably expressed among individual isolates of FeLV derived from different cat lymphoma cell lines.