Determination of hydrogen peroxide in a flow system with microperoxidase as catalyst for the luminol chemiluminescence reaction
Determination of hydrogen peroxide in a flow system with microperoxidase as catalyst for the luminol chemiluminescence reaction
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以微过氧化物酶为鲁米诺化学发光反应催化剂的流动系统中过氧化氢的测定
DOI：
10.1016/s0003-2670(01)95369-3
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发表时间：
1982
影响因子：
6.2
通讯作者：
B. Olsson
中科院分区：
文献类型：
--
作者：
B. Olsson
过氧化氢采用化学发光法测定，试剂为100 μM鲁米诺和3 μM微过氧化物酶，溶液pH为10（碳酸盐缓冲液）。微过氧化物酶作为催化剂优于血红素。该方法使用自动流动注射系统，每分钟的吞吐量为2个样品。对数-对数校准图从检出限开始呈线性（斜率为1.3），3 × 10-9M至10-5M H2O2。背景辐射很低。除非采取预防措施，否则载体流和某些塑料中的杂质可能导致本底升高。
Hydrogen peroxide is determined by a chemiluminescence method with a reagent containing 100 μM luminol and 3 μM microperoxidase at pH 10 (carbonate buffer). Microperoxidase is superior to hematin as a catalyst. The method uses an automated flow injection system with a throughput of 2 samples per minute. The log—log calibration plot is linear (slope 1.3) from the detection limit, 3 × 10-9M up to 10-5M H2O2. The background emission is low. Impurities in the carrier stream and from some plastics may cause elevated background unless precautions are taken.