COMPARISON OF INTRAVENOUS GAMMA-GLOBULIN AND A MONOCLONAL ANTI-FC RECEPTOR ANTIBODY AS INHIBITORS OF IMMUNE CLEARANCE INVIVO IN MICE
COMPARISON OF INTRAVENOUS GAMMA-GLOBULIN AND A MONOCLONAL ANTI-FC RECEPTOR ANTIBODY AS INHIBITORS OF IMMUNE CLEARANCE INVIVO IN MICE
复制标题
DOI：
10.1172/jci112530
复制
发表时间：
1986-06-01
影响因子：
15.9
通讯作者：
HALL, J
中科院分区：
文献类型：
--
作者：
KURLANDER, RJ;HALL, J
将单体人IgG、热聚集人IgG和单克隆抗小鼠巨噬细胞Fc II受体抗体（2，4G 2）输注至正常小鼠后，评估Fc受体介导的清除率和非特异性吞噬细胞清除率。每种药剂都在体内阻断Fc受体功能，但每微克2.4G2比其他药剂更有效。阻断剂量的单体IgG不影响免疫功能的其他方面。相比之下，聚集的IgG以及2.4G2在较小程度上降低了血清补体水平。此外，这些药物还导致非特异性吞噬功能中度降低。单克隆抗小鼠巨噬细胞C3 bi受体抗体（Mac-1），另一种结合巨噬细胞CR 3受体而不干扰Fc-受体功能的单克隆抗体，也可降低血清补体并抑制非特异性吞噬功能。单独的补体耗竭（通过输注眼镜蛇毒因子产生）不能解释观察到的Fc受体或非特异性吞噬功能的变化。我们得出结论，单体IgG和抗Fc受体抗体可以显着抑制Fc受体功能在体内，但是，这些代理商产生的生理变化的模式不同。
Fc-receptor-mediated clearance and nonspecific phagocytic clearance were assessed after the infusion of monomeric human IgG, heat-aggregated human IgG, and a monoclonal anti-mouse macrophage FcII receptor antibody (2,4G2) into normal mice. Each agent blocked Fc-receptor function in vivo, but 2.4G2 was much more potent per microgram than the other agents. Monomeric IgG in blocking doses did not affect other aspects of immune function. In contrast, aggregated IgG, and to a lesser extent, 2.4G2 reduced serum complement levels. In addition, these agents also caused moderate reductions in nonspecific phagocytic function. Monoclonal anti-mouse macrophage C3bi receptor antibody (Mac-1), another monoclonal antibody which binds to macrophage CR3 receptors without interfering with Fc-receptor function, also reduced serum complement and inhibited nonspecific phagocytic function. Complement depletion alone (produced by infusion of cobra venom factor) could not account for the observed changes in Fc receptor or nonspecific phagocytic function. We conclude that both monomeric IgG and anti-Fc-receptor antibodies can markedly inhibit Fc-receptor function in vivo; however, the pattern of physiologic changes produced by these agents differs.