Immunodetection of noncollagenous matrix proteins during periodontal tissue regeneration.
Immunodetection of noncollagenous matrix proteins during periodontal tissue regeneration.
复制标题
牙周组织再生过程中非胶原基质蛋白的免疫检测。
DOI：
10.1034/j.1600-0765.2001.036004205.x
复制
发表时间：
2001
影响因子：
3.5
通讯作者：
A. Nanci
中科院分区：
文献类型：
--
作者：
H. Kawaguchi;T. Ogawa;H. Kurihara;A. Nanci
本研究采用大鼠牙槽骨缺损模型，应用形态学和免疫细胞化学方法研究了脱层牙本质与再生牙周组织之间的界面。沿着上颌第一磨牙的腭根通过手术沿着暴露牙本质表面。处理后3周，对动物进行灌注，并将脱钙下颌骨的处理区域包埋在Epon中进行超微结构研究或LR白色中进行包埋后免疫金标记。将薄组织切片与抗非胶原基质（骨桥蛋白、骨唾液蛋白、骨钙蛋白和纤连蛋白）和血浆（α 2 HS-糖蛋白和白蛋白）蛋白的抗体孵育。虽然在某些情况下，再生事件直接发生在裸露的牙本质表面上，但裸露的牙本质和再生牙周组织之间的界面经常以界面区的存在为特征。该区域有时显示出电子致密的、水泥线状的、平面的骨桥蛋白和骨唾液蛋白免疫反应性有机物质的积聚。骨钙素和α 2 HS-糖蛋白的免疫标记是温和的，弥漫在整个界面区，而与白蛋白和纤连蛋白的抗体标记导致弱反应。它的结论是，骨唾液酸蛋白和骨桥蛋白的积累是一个主要的事件，在形成再生牙骨质裸露的根面。
The interface between denuded dentin and regenerative periodontal tissue was investigated in a rat alveolar bone defect model using morphological and immunocytochemical approaches. The dentin surface was surgically exposed along the palatal roots of maxillary first molars. At 3 weeks post treatment, animals were perfused and treated regions from decalcified mandibles were embedded in Epon for ultrastructural studies or LR White for post-embedding immunogold labeling. Thin tissue sections were incubated with antibodies against noncollagenous matrix (osteopontin, bone sialoprotein, osteocalcin and fibronectin) and plasma (alpha2HS-glycoprotein and albumin) proteins. While in some cases, regenerative events took place directly on the denuded dentin surface, the interface between the denuded dentin and regenerating periodontal tissue was frequently characterized by the presence of an interfacial zone. This zone sometimes showed an electron-dense, cement line-like, planar accumulation of organic material immunoreactive for osteopontin and bone sialoprotein. Immunolabeling for osteocalcin and alpha2HS-glycoprotein was moderate and diffuse throughout the interfacial zone, whereas labeling with antibodies to albumin and fibronectin resulted in a weak reaction. It is concluded that accumulation of bone sialoprotein and osteopontin is a primary event during the formation of regenerative cementum onto denuded root surfaces.