Isolation and in vitro expansion of human colonic stem cells
Isolation and in vitro expansion of human colonic stem cells
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DOI：
10.1038/nm.2470
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发表时间：
2011-10-01
期刊：
影响因子：
82.9
通讯作者：
Batlle, Eduard
中科院分区：
文献类型：
--
作者：
Jung, Peter;Sato, Toshiro;Batlle, Eduard
在这里，我们描述了人类结肠上皮干细胞的分离。不同细胞表面丰富的肾上腺素B型受体2(EPHB2)允许从人结肠粘膜活检组织中纯化不同类型的细胞。最高的EPHB2表面水平对应于端粒最长的结肠上皮细胞和肠道干细胞(ISC)标记基因的高表达。此外，使用重建ISC利基的培养条件，相当大比例的EPHB2高表达细胞可以在体外作为未分化和多潜能的群体进行扩增。
Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population.