Alterations in sperm-inherited noncoding RNAs associate with late-term fetal growth restriction induced by preconception paternal alcohol use
Alterations in sperm-inherited noncoding RNAs associate with late-term fetal growth restriction induced by preconception paternal alcohol use
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DOI：
10.1016/j.reprotox.2019.04.006
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发表时间：
2019-08-01
影响因子：
3.3
通讯作者：
Golding, Michael C.
中科院分区：
文献类型：
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作者：
Bedi, Yudhishtar;Chang, Richard C.;Golding, Michael C.
使用小鼠模型，我们的研究小组最近描述了父亲在怀孕前长期饮酒与后代生长缺陷之间的关联。在这里，我们试图确定酒精暴露对男性生殖生理的影响，以及精子遗传的非编码RNA与观察到的生长缺陷的传播之间的关系。酒精暴露没有明显改变男性生殖生理或生育能力。然而，长期饮酒可重复地诱导后代的晚期胎儿生长受限，这与转移RNA衍生的小RNA与Piwi相互作用RNA的比例比率的变化以及暴露于酒精的精子中microRNA miR21，miR30和miR142的富集改变相关。虽然我们的数据集与先前研究父系压力对后代表型影响的工作有相似之处，但我们无法确定血浆皮质酮的任何变化，表明酒精可能通过不同的机制改变精子遗传的非编码RNA。
Using a mouse model, our group recently described an association between chronic paternal alcohol use prior to conception and deficits in offspring growth. Here, we sought to determine the impact of alcohol exposure on male reproductive physiology and the association of sperm-inherited noncoding RNAs with the transmission of the observed growth defects. Alcohol exposure did not appreciably alter male reproductive physiology or fertility. However, chronic alcohol use reproducibly induced late-term fetal growth restriction in the offspring, which correlated with a shift in the proportional ratio of transfer RNA-derived small RNAs to Piwi-interacting RNAs, as well as altered enrichment of microRNAs miR21, miR30, and miR142 in alcohol-exposed sperm. Although our dataset share similarities to prior works examining the impact of paternal stress on offspring phenotype, we were unable to identify any changes in plasma corticosterone, indicating alcohol may alter sperm-inherited noncoding RNAs through distinct mechanisms.