Transcriptional profiling of Mycoplasma hyopneumoniae during iron depletion using microarrays
Transcriptional profiling of Mycoplasma hyopneumoniae during iron depletion using microarrays
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DOI：
10.1099/mic.0.28674-0
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发表时间：
2006-04-01
期刊：
影响因子：
2.8
通讯作者：
Minion, FC
中科院分区：
文献类型：
--
作者：
Madsen, ML;Nettleton, D;Minion, FC
猪肺炎支原体是猪流行性肺炎的病原体，也是猪呼吸道疾病综合征的主要组成部分，尽管在世界范围内开展了控制计划，但它仍然困扰着猪生产者。该疾病是慢性和自限性的，但宿主易发生免疫病理学变化，从而增强与其他病原体相关的呼吸道疾病。M的反应。由于其与其他细菌病原体中的毒力机制相关，因此对环境压力的低致病性是令人感兴趣的。这些应激源之一，缺铁，是宿主先天免疫反应的一个突出特征，并且肯定会影响支原体在体内的生长。为了研究这一点，我们将基因芯片技术应用于M.缺铁期间的低血压。使用由基因组中698个ORF中的632个组成的阵列来比较从在正常实验室条件下生长的生物体中分离的mRNA与从用螯合剂2，2 '-联吡啶进行铁剥夺的生物体中分离的mRNA。该分析确定了27个基因在低铁生长条件下上调或下调（P < 0.01），估计错误发现率低于10%。这些基因包括编码转运蛋白、参与能量代谢的酶和翻译过程的组分的基因。27个基因中有10个没有指定的功能。这些研究表明，M. hypoprotein可以响应环境条件的变化，但其机制仍然未知。
Mycoplasma hyopneumoniae, the causative agent of swine enzootic pneumonia and a major component of the porcine respiratory disease complex, continues to confound swine producers despite control programmes worldwide. The disease is chronic and self-limiting, but the host is subject to immunopathological changes that potentiate respiratory disease associated with other pathogens. The response of M. hyopneumoniae to environmental stress is of interest because of its relevance to virulence mechanisms in other bacterial pathogens. One of these stressors, iron deprivation, is a prominent feature of the host innate immune response, and most certainly impacts growth of mycoplasmas in vivo. To study this, microarray technology was applied to the transcriptome analysis of M. hyopneumoniae during iron deprivation. An array consisting of 632 of the 698 ORFs in the genome was used to compare the mRNA isolated from organisms grown under normal laboratory conditions with that from organisms subjected to iron deprivation with the chelator 2,2 '-dipyridyl. This analysis identified 27 genes that were either up- or down-regulated in response to low-iron growth conditions (P < 0.01), with an estimated false discovery rate below 10 %. These included genes encoding transport proteins, enzymes involved in energy metabolism, and components of the translation process. Ten of the 27 identified genes had no assigned function. These studies indicate that M. hyopneumoniae can respond to changes in environmental conditions, but the mechanism employed remains unknown.