Comparison of historical and contemporary isolates of Senecavirus A
Comparison of historical and contemporary isolates of Senecavirus A
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DOI：
10.1016/j.vetmic.2020.108946
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发表时间：
2021-02-01
影响因子：
3.3
通讯作者：
Lager, Kelly M.
中科院分区：
文献类型：
--
作者：
Buckley, Alexandra C.;Michael, David D.;Lager, Kelly M.
塞内卡病毒A （SVA）于2002年被发现是一种细胞培养污染物，多次尝试通过实验复制疾病都没有成功。除了2015年巴西和美国爆发的猪特发性水疱病（PIVD）病例外，对SVA检测PCR阳性的猪特发性水疱病（PIVD）病例的现场报告表明，SVA是一种病原体，现在已经得到了一致的实验证明。当代SVA菌株的疾病实验再现的便便性提出了有关历史分离株再现水疱病的困难的问题。下面进行的研究比较了生长猪中SVA的历史和现代分离株的致病性。6组猪（n = 8）经鼻接种SVV001/2002、CAN/2011、HI/2012、IA/2015、NC/2015、SD/2015分离株。所有分离株在每组至少一半的接种猪中引起水疱病。通过定量PCR检测SVA阳性的血清和/或拭子样本证实所有猪都复制了病毒。病毒中和试验显示猪血清对研究中使用的所有病毒具有交叉中和抗体。接种历史分离株的猪的交叉中和抗体低于接种当代分离株的猪。系统发育分析显示，与其他5个分离株相比，SVV001/2002的两个进化支位于单独的Glade。虽然这6株分离株的感染动力学和序列存在差异，但水疱病的临床表现在历史和当代分离株之间相似。
Senecavirus A (SVA) was discovered as a cell culture contaminant in 2002, and multiple attempts to experimentally reproduce disease were unsuccessful. Field reports of porcine idiopathic vesicular disease (PIVD) cases testing PCR positive for SVA in addition to outbreaks of PIVD in Brazil and the United States in 2015 suggested SVA was a causative agent, which has now been consistently demonstrated experimentally. Ease of experimental reproduction of disease with contemporary strains of SVA raised questions concerning the difficulty of reproducing vesicular disease with historical isolates. The following study was conducted to compare the pathogenicity of SVA between historical and contemporary isolates in growing pigs. Six groups of pigs (n = 8) were intranasally inoculated with the following SVA isolates: SVV001/2002, CAN/2011, HI/2012, IA/2015, NC/2015, SD/2015. All isolates induced vesicular disease in at least half of the inoculated pigs from each group. All pigs replicated virus as demonstrated by serum and/or swab samples positive for SVA by quantitative PCR. Pig sera tested by virus neutralization assay demonstrated cross-neutralizing antibodies against all viruses utilized in the study. Cross-neutralizing antibodies from pigs inoculated with historical isolates were lower than those pigs that were inoculated with contemporary isolates. Phylogenetic analysis revealed two clades with SVV001/2002 being in a separate Glade compared to the other five isolates. Although differences in the infection kinetics and sequences of these six isolates were found, clinical presentation of vesicular disease was similar between both historical and contemporary isolates.