Canine coronavirus infections in Japan: Virological and epidemiological aspects
Canine coronavirus infections in Japan: Virological and epidemiological aspects
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DOI：
10.1292/jvms.61.731
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发表时间：
1999-07-01
影响因子：
1.2
通讯作者：
Mochizuki, M
中科院分区：
文献类型：
--
作者：
Bandai, C;Ishiguro, S;Mochizuki, M
对犬冠状病毒（canine coronavirus， CCV） 10株、8株现场株和2株参考实验室株的抗原性关系和致病性进行了比较研究。对猫冠状病毒单克隆抗体和CCV高免疫抗血清进行中和和免疫荧光检测，发现各菌株之间存在不同程度的抗原多样性，但认为它们属于同一血清型。实验中，接种不同CCV毒株的无特定病原体幼犬表现出临床症状，但其毒力存在差异。为了阐明日本犬中CCV感染的流行情况，我们对467只野地犬进行了CCV中和抗体检测，发现其患病率为44.1%。此外，通过对最近在兽医诊所就诊的100只腹泻犬的直肠棉签进行巢式逆转录-聚合酶链反应，发现16%的样本中存在CCV的证据。结果表明，CCV感染在犬中比预期的更广泛，并且CCV也是日本犬腹泻的重要病因。
Ten strains, eight field and two reference laboratory strains, of canine coronavirus (CCV) were comparatively examined with respect to antigenic relationships and pathogenic potential in dogs. With monoclonal antibodies and hyperimmune antisera to feline coronavirus and CCV, respectively, varying degrees of antigenic diversities were found among the strains by neutralization and immunofluorescence assays, but it was felt that they belong to one serotype. Specific-pathogen-free puppies experimentally inoculated with some CCV strains manifested clinical symptoms, but there was a difference in their virulence. In order to elucidate the prevalence of CCV infections in dogs in Japan, we tested for neutralizing antibodies to CCV in 467 field dogs, and found a prevalence of 44.1%. Moreover, by using nested reverse transcriptase-polymerase chain reaction on rectal swabs of 100 diarrheic dogs recently presented in veterinary clinics, evidence of CCV in 16% of these specimens was found. The results suggested that CCV infection is more widespread than expected in dogs, and that CCV is a significant etiologic factor in canine diarrhea also in Japan.