DEAP1 encodes a fasciclin-like arabinogalactan protein required for male fertility in rice
DEAP1 encodes a fasciclin-like arabinogalactan protein required for male fertility in rice
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DEAP1 编码水稻雄性生育能力所需的类成束蛋白阿拉伯半乳聚糖蛋白
DOI：
10.1111/jipb.13271
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发表时间：
2022-06-02
影响因子：
11.4
通讯作者：
Li, Shuangcheng
中科院分区：
文献类型：
--
作者：
Zhou, Dan;Zou, Ting;Li, Shuangcheng
阿拉伯半乳糖蛋白(AGPs)广泛存在于植物细胞中。Fasciclin-like AGPs(FLAs)属于AGPs的一个亚类，在植物的生长发育中发挥重要作用。然而，人们对水稻Fla的生物学功能知之甚少。在此，我们报道了一个水稻缺陷型ExINE和开孔PATTERNING1(DEAP1)雄性不育突变体的鉴定。Deap1突变体的花药产生异常的花粉粒，外壁形成缺陷，气孔环变平，绒被层退化略有延迟。DEAP1编码第三类植物FLAs的一个与质膜相关的成员，在雄性发育过程中在生殖细胞和绒毛层中特异和暂时表达。基因表达研究显示，在deap1突变体中，与外壁形成、孔道形成和绒毡层发育相关的基因转录积累减少。此外，DEAP1可能与两个水稻D6蛋白激酶-LIKE3s(OsD6PKL3s)相互作用，影响水稻花粉孔发育。我们的发现表明，DEAP1参与了雄性生殖发育，并可能影响外壁形成和孔道模式，从而为了解植物FLA在雄性可育性中的分子功能提供了新的见解。
Arabinogalactan proteins (AGPs) are widely distributed in plant cells. Fasciclin-like AGPs (FLAs) belong to a subclass of AGPs that play important roles in plant growth and development. However, little is known about the biological functions of rice FLA. Herein, we report the identification of a male-sterile mutant of DEFECTIVE EXINE AND APERTURE PATTERNING1 (DEAP1) in rice. The deap1 mutant anthers produced aberrant pollen grains with defective exine formation and a flattened aperture annulus and exhibited slightly delayed tapetum degradation. DEAP1 encodes a plasma membrane-associated member of group III plant FLAs and is specifically and temporally expressed in reproductive cells and the tapetum layer during male development. Gene expression studies revealed reduced transcript accumulation of genes related to exine formation, aperture patterning, and tapetum development in deap1 mutants. Moreover, DEAP1 may interact with two rice D6 PROTEIN KINASE-LIKE3s (OsD6PKL3s), homologs of a known Arabidopsis aperture protein, to affect rice pollen aperture development. Our findings suggested that DEAP1 is involved in male reproductive development and may affect exine formation and aperture patterning, thereby providing new insights into the molecular functions of plant FLAs in male fertility.