Within-Host Recombination in the Foot-and-Mouth Disease Virus Genome.
Within-Host Recombination in the Foot-and-Mouth Disease Virus Genome.
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DOI：
10.3390/v10050221
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发表时间：
2018-04-25
期刊：
影响因子：
--
通讯作者：
Ribeca P
中科院分区：
文献类型：
--
作者：
Ferretti L;Di Nardo A;Singer B;Lasecka-Dykes L;Logan G;Wright CF;Pérez-Martín E;King DP;Tuthill TJ;Ribeca P
口蹄疫病毒（FMDV）的遗传多样性的决定因素之一是变异。FMDV序列具有由广泛的血清型内和血清型间重组引起的嵌合结构，除了编码衣壳蛋白的区域。虽然这些基因组范围的大规模重组模式得到了很好的研究，但对感染宿主内可能存在的重组模式知之甚少。此外，由于序列的相似性和区分重组与点突变的局限性，检测在宿主内水平进化的病毒之间的重组具有挑战性。在这里，我们提出了密切相关的口蹄疫病毒序列发生在布法罗主机之间的重组事件的第一次分析。这些事件的检测是可能的两个病毒群的共感染与约1%的核苷酸分歧的发生。我们发现了超过15个重组事件，不均匀地分布在来自不同动物的8个样本中。这些事件的分布沿着口蹄疫病毒基因组既不均匀，也不相关的重组断点的系统发育分布，这表明宿主内的进化压力和长期选择的感染性和传播性之间的不匹配。
Recombination is one of the determinants of genetic diversity in the foot-and-mouth disease virus (FMDV). FMDV sequences have a mosaic structure caused by extensive intra- and inter-serotype recombination, with the exception of the capsid-encoding region. While these genome-wide patterns of broad-scale recombination are well studied, not much is known about the patterns of recombination that may exist within infected hosts. In addition, detection of recombination among viruses evolving at the within-host level is challenging due to the similarity of the sequences and the limitations in differentiating recombination from point mutations. Here, we present the first analysis of recombination events between closely related FMDV sequences occurring within buffalo hosts. The detection of these events was made possible by the occurrence of co-infection of two viral swarms with about 1% nucleotide divergence. We found more than 15 recombination events, unequally distributed across eight samples from different animals. The distribution of these events along the FMDV genome was neither uniform nor related to the phylogenetic distribution of recombination breakpoints, suggesting a mismatch between within-host evolutionary pressures and long-term selection for infectivity and transmissibility.
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4.8
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通讯作者：
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