YEAST GLOBAL TRANSCRIPTIONAL REGULATORS SIN4 AND RGR1 ARE COMPONENTS OF MEDIATOR COMPLEX RNA-POLYMERASE-II HOLOENZYME
YEAST GLOBAL TRANSCRIPTIONAL REGULATORS SIN4 AND RGR1 ARE COMPONENTS OF MEDIATOR COMPLEX RNA-POLYMERASE-II HOLOENZYME
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DOI：
10.1073/pnas.92.24.10864
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发表时间：
1995-11-21
影响因子：
11.1
通讯作者：
KORNBERG, RD
中科院分区：
文献类型：
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作者：
LI, Y;BJORKLUND, S;KORNBERG, RD
Sin 4和Rgr 1蛋白，以前遗传学研究显示，在许多基因的转录调控中发挥积极和消极的作用，在这里被确定为介质和RNA聚合酶II全酶复合物的组成部分。Sin4缺失和Rgr 1截短菌株的结果表明，这些蛋白质在一个亚复合物，包括Sin4，Rgr 1，Gal 11，和一个50 kDa的多肽的协会。与以前的遗传学证据一起，我们的研究结果指出了介体在抑制和转录激活中的作用。
Sin4 and Rgr1 proteins, previously shown by genetic studies to play both positive and negative roles in the transcriptional regulation of many genes, are identified here as components of mediator and RNA polymerase II holoenzyme complexes. Results with Sin4 deletion and Rgr1 truncation strains indicate the association of these proteins in a subcomplex comprising Sin4, Rgr1, Gal11, and a 50-kDa polypeptide. Taken together with the previous genetic evidence, our findings point to a role of the mediator in repression as well as in transcriptional activation.