Application of a new zwitterionic hydrophillic interaction chromatography column for determination of paralytic shellfish poisoning toxins
Application of a new zwitterionic hydrophillic interaction chromatography column for determination of paralytic shellfish poisoning toxins
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DOI：
10.1002/jssc.200700025
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发表时间：
2007-08-01
影响因子：
3.1
通讯作者：
Luckas, Bernd
中科院分区：
文献类型：
--
作者：
Diener, Marc;Erler, Katrin;Luckas, Bernd
提出了一种基于两性离子亲水作用色谱柱(HILIC)分离麻痹性贝毒毒素的新方法。极性PSP毒素在ZIC-HILIC柱上的有效保留使其能够通过应用质谱学(MS/MS)检测或在荧光检测(FD)之前氧化后作为衍生物选择性和灵敏地测定。较低的缓冲液浓度和离子对试剂的省略降低了MS/MS分析的检出限，两种方法均表现出良好的线性关系。该方法可用于各种浮游植物中PSP毒素的定性和定量检测，也可用于海产品的常规分析。
A novel method for paralytic shellfish poisoning (PSP) toxins which is based on the chromatographic separation of the toxins using a zwitterionic (ZIC) hydrophilic interaction chromatography (HILIC) column is presented. Efficient retention of the polar PSP toxins on the ZIC-HILIC column allowed their selective and sensitive determination by the application of mass spectrometric (MS/MS) detection or as derivatives after oxidation prior to fluorescence detection (FD). Low buffer concentrations and the omission of ion-pair reagents decreased the limits of detection (LODs) by MS/MS analysis and showed a good linearity for both methods of detection. This method can be applied for the qualitative and quantitative determination of PSP toxins in various types of phytoplankton, and for the routine analysis of seafood.