INTRACEREBROVENTRICULAR INFUSIONS OF 3-OHB AND INSULIN IN A RAT MODEL OF DIETARY OBESITY
INTRACEREBROVENTRICULAR INFUSIONS OF 3-OHB AND INSULIN IN A RAT MODEL OF DIETARY OBESITY
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DOI：
10.1152/ajpregu.1988.255.6.r974
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发表时间：
1988-12-01
影响因子：
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通讯作者：
BRAY, GA
中科院分区：
文献类型：
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作者：
ARASE, K;FISLER, JS;BRAY, GA
我们研究了膳食脂肪对3-羟基丁酸（3-OHB）和胰岛素长期输注到三种不同品系的大鼠第三脑室中的反应的影响，这些大鼠对高脂饮食诱导的肥胖具有不同的易感性：Osdelet-Mendel大鼠最敏感; Sprague-Dawley大鼠居中; S5 B/Pl大鼠最抵抗。将套管植入第三脑室10天后，大鼠喂食低脂肪或高脂肪饮食14天。在第7天，将渗透微型泵连接到心室插管。3-OHB输注（3.6 μ mol/24 h）降低了食用任一饮食的Sprague-Dawley和Osborne-Mendel大鼠的食物摄入和体重。饮食性脂肪抵抗的S5 B/P1大鼠对侧脑室注射3-OHB无反应。输注胰岛素（10 mU/24 h）降低了食用低脂（高碳水化合物）饮食的动物的摄食量和体重，但在食用高脂肪饮食的动物中没有。饮食严重影响对脑室内胰岛素输注的反应，但对3-OHB的反应没有影响。
We examined the effect of dietary fat on the response to 3-hydroxybutyrate (3-OHB) and insulin infused chronically into the third ventricle in three strains of rats with differing susceptibility to obesity induced by a high-fat diet: Osborne-Mendel rats are most susceptible; Sprague-Dawley rats are intermediate; and S5B/Pl rats are most resistant. Ten days after implantation of cannulas into the third ventricle, rats were fed either a low-diet or a high-fat diet for 14 days. On day 7, osmotic minipumps were attached to the ventricular cannulus. 3-OHB infusions (3.6 .mu.mol/24 h) reduced food intake and body weight in Sprague-Dawley and Osborne-Mendel rats eating either diet. The dietary fat-resistant S 5B/Pl rats did not respond to the intracerebroventricular infusion of 3-OHB. The infusion of insulin (10 mU/24 h) lowered food intake and body weight in animals eating the low-fat (high-carbohydrate) diet but not in animals eating the high-fat diet. Diet profoundly affects the response to intracerebroventricular infusions of insulin but is without effect on the response to 3-OHB.