Plant Growth Promotion by Volatile Organic Compounds Produced by Bacillus subtilis SYST2.
Plant Growth Promotion by Volatile Organic Compounds Produced by Bacillus subtilis SYST2.
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枯草芽孢杆菌 SYST2 产生的挥发性有机化合物促进植物生长
DOI：
10.3389/fmicb.2017.00171
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发表时间：
2017
影响因子：
5.2
通讯作者：
Gao X
中科院分区：
文献类型：
--
作者：
Tahir HA;Gu Q;Wu H;Raza W;Hanif A;Wu L;Colman MV;Gao X
细菌挥发物通过调节植物激素的合成或代谢而在促进植物生长方面发挥重要作用。在室内和生长室内试验中，研究了植物生长促生菌枯草芽孢杆菌SYST2菌株产生的挥发性有机化合物(VOCs)对番茄植株激素调节和生长促进的影响。我们观察到在这两个实验条件下植物生物量显著增加；我们观察到光合作用和内源赤霉素、生长素和细胞分裂素含量的增加，而乙烯水平则下降。通过气相色谱-质谱法对SYST2排放的VOCs进行了鉴定。在装有试管植物的玻璃瓶中测试的11种VOCs中，只有两种--沙丁胺醇和1，3-丙二醇--被发现能促进植物生长。此外，番茄植株表现出与生长素相关的基因(SlIAA1)的差异表达。SLAA3)、赤霉素(GA20ox-1)、细胞分裂素(SlCKX1)、扩展蛋白(Exp2、Exp9)。Exp 18)，以及根和叶中乙烯(ACO1)的生物合成或代谢。枯草杆菌SYST2挥发性有机物。我们的发现表明，SYST2来源的VOCs通过触发生长激素活性来促进植物生长，并为细菌VOCs促进植物生长的机制提供了新的见解。
Bacterial volatiles play a significant role in promoting plant growth by regulating the synthesis or metabolism of phytohormones.In vitroand growth chamber experiments were conducted to investigate the effect of volatile organic compounds (VOCs) produced by the plant growth promoting rhizobacteriumBacillus subtilisstrain SYST2 on hormone regulation and growth promotion in tomato plants. We observed a significant increase in plant biomass under both experimental conditions; we observed an increase in photosynthesis and in the endogenous contents of gibberellin, auxin, and cytokinin, while a decrease in ethylene levels was noted. VOCs emitted by SYST2 were identified through gas chromatography-mass spectrometry analysis. Of 11 VOCs tested in glass jars containing plants in test tubes, only two, albuterol and 1,3-propanediole, were found to promote plant growth. Furthermore, tomato plants showed differential expression of genes involved in auxin (SlIAA1. SlIAA3), gibberellin (GA20ox-1), cytokinin (SlCKX1), expansin (Exp2, Exp9. Exp 18), and ethylene (ACO1) biosynthesis or metabolism in roots and leaves in response toB. subtilisSYST2 VOCs. Our findings suggest that SYST2-derived VOCs promote plant growth by triggering growth hormone activity, and provide new insights into the mechanism of plant growth promotion by bacterial VOCs.