Contamination status and health risk assessment of 31 mycotoxins in six edible and medicinal plants using a novel green defatting and depigmenting pretreatment coupled with LC-MS/MS
Contamination status and health risk assessment of 31 mycotoxins in six edible and medicinal plants using a novel green defatting and depigmenting pretreatment coupled with LC-MS/MS
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采用新型绿色脱脂脱色预处理结合 LC-MS/MS 对 6 种食用和药用植物中 31 种霉菌毒素的污染状况和健康风险评估
DOI：
10.1016/j.lwt.2022.113401
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发表时间：
2022-04-01
影响因子：
6
通讯作者：
Yang, Mei-Hua
中科院分区：
文献类型：
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作者：
Lu, Qian;Ruan, Hao-Nan;Yang, Mei-Hua
食用和药用植物种子中的真菌毒素污染是消费者关注的潜在毒理学问题。首次建立了一种新型、绿色、高效的脱脂脱色净化前处理方法，并将其应用于31种真菌毒素的冷冻固相萃取同时测定。冷冻提取和固相萃取相结合，可以达到脱脂和脱色的双重净化效果。两者结合，可以达到双重清理效果。方法验证、日间和日内精密度（RSD < 10%）和回收率（79.5-125.1%）的性能特征优异，符合现行法规。令人震惊的是，在117批样品中，超过30%的样品含有一种以上的真菌毒素，其中7.69%的样品被检测出含量超过欧洲药典规定的限度，其中4种样品被检测出含量超过最大残留量的5倍。此外，两种风险评估方法确定黄曲霉毒素和玉米赤霉烯酮是这些基质中最具风险诱导性的真菌毒素。本文首次报道了6种食药用植物种子（薏苡仁、桃仁、酸枣仁、决明子、麦芽和肉豆蔻）中多种真菌毒素的污染状况及风险评价。该方法对于评价食用和药用植物种子或其他富含油脂基质中的多种真菌毒素暴露具有独特的优势。
Mycotoxin contamination in edible and medicinal plants seeds is a potential toxicological concern for consumers. A novel, green and efficient defatting and de-pigment clean-up pretreatment was first developed for the simultaneous determination of 31 mycotoxins via freeze-out combined with solid-phase extraction. Combination of freeze-out and solid-phase extraction can achieve a double clean-up effect: defatting and depigmenting. The combination of the two can achieve a double clean-up effect. Excellent performance characteristics of method validation, inter-and intra-day precision (RSD < 10%), and recovery (79.5-125.1%) were obtained, fulfilling the current legislation. Alarmingly, of the 117 batches of samples, more than 30% contained more than one kind of mycotoxin, 7.69% of which were detected at levels exceeding the limits defined in the European Pharmacopoeia, and four of which were detected at levels over five times higher than the maximum residue level. Furthermore, two risk assessment methods identified aflatoxins and Zearalenone as the most risk-inducing mycotoxins in these matrices. This is the first report on the contamination status and risk assessment of multi-mycotoxins in six edible and medicinal plant seeds (Coicis Semen, Semen Perisicae, Ziziphi Spinosae Semen, Cassiae Semen, malt and nutmeg). The presented method has unique advantages for evaluating multi-mycotoxin exposure in edible and medicinal plant seeds or other oil-rich matrices.