Dynamics of thymocyte-stromal cell interactions visualized by two-photon microscopy
Dynamics of thymocyte-stromal cell interactions visualized by two-photon microscopy
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DOI：
10.1126/science.1070945
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发表时间：
2002-06-07
期刊：
影响因子：
56.9
通讯作者：
Robey, E
中科院分区：
文献类型：
--
作者：
Bousso, P;Bhakta, NR;Robey, E
根据胸腺细胞与胸腺基质上显示的自身主要组织相容性复合体(MHC)-肽复合体相互作用的能力，选择胸腺细胞成熟。使用双光子显微镜，我们实时分析了在三维胸腺器官培养中，通过阳性选择发育的胸腺细胞所形成的细胞接触。在这些培养物中，有很大一部分胸腺细胞是高度能动的。研究发现，MHC识别可延长胸腺细胞-基质细胞相互作用的持续时间，并以显示稳定的细胞-细胞接触的长寿命细胞联系和较短的、高度动态的联系发生。我们的结果确定了胸腺细胞在正选择过程中相互作用的多样性和动力学。
Thymocytes are selected to mature according to their ability to interact with self major histocompatibility complex (MHC)-peptide complexes displayed on the thymic stroma. Using two-photon microscopy, we performed real-time analysis of the cellular contacts made by developing thymocytes undergoing positive selection in a three-dimensional thymic organ culture. A large fraction of thymocytes within these cultures were highly motile. MHC recognition was found to increase the duration of thymocyte-stromal cell interactions and occurred as both long-lived cellular associations displaying stable cell-cell contacts and as shorter, highly dynamic contacts. Our results identify the diversity and dynamics of thymocyte interactions during positive selection.