Investigating multi-material hydrogel three-dimensional printing for in vitro representation of the neo-vasculature of solid tumours: a comprehensive mechanical analysis and assessment of nitric oxide release from human umbilical vein endothelial cells.
Investigating multi-material hydrogel three-dimensional printing for in vitro representation of the neo-vasculature of solid tumours: a comprehensive mechanical analysis and assessment of nitric oxide release from human umbilical vein endothelial cells.
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DOI：
10.1098/rsos.230929
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发表时间：
2023-08
影响因子：
3.5
通讯作者：
Domingo-Roca, Roger
中科院分区：
文献类型：
--
作者：
Asciak, Lisa;Gilmour, Lauren;Williams, Jonathan A.;Foster, Euan;Diaz-Garcia, Lara;McCormick, Christopher;Windmill, James F. C.;Mulvana, Helen E.;Jackson-Camargo, Joseph C.;Domingo-Roca, Roger
关键词：
许多实体瘤（例如肉瘤、癌和淋巴瘤）形成紊乱的新血管系统，启动不受控制的血管形成以支持肿瘤生长。这些环境的复杂性对肿瘤医学研究提出了重大挑战。虽然动物模型通常用于解决其中一些挑战，但它们既耗时又引起伦理问题。人们已经探索了体外微生理系统作为替代方案，但其生产通常需要多步骤光刻工艺，这限制了其生产。在这项工作中，通过立体光刻三维打印研究了一种快速开发多材料组织模拟、细胞兼容平台的新方法，该平台能够代表实体肿瘤新脉管系统的复杂性。为此，我们设计并表征了一系列丙烯酸酯树脂，这些树脂可产生具有健康和患病机械声组织模拟特性的共价光交联水凝胶。通过研究人脐静脉内皮细胞的形态、肌动蛋白表达、粘着斑和一氧化氮释放，评估这些材料在临床前研究中取代动物测试的潜在可行性。这些材料被用来生产实体瘤新脉管系统的简化多材料三维打印模型，证明了我们的方法在体外复制实体瘤复杂性的潜力，而无需进行动物测试。
Many solid tumours (e.g. sarcoma, carcinoma and lymphoma) form a disorganized neo-vasculature that initiates uncontrolled vessel formation to support tumour growth. The complexity of these environments poses a significant challenge for tumour medicine research. While animal models are commonly used to address some of these challenges, they are time-consuming and raise ethical concerns. In vitro microphysiological systems have been explored as an alternative, but their production typically requires multi-step lithographic processes that limit their production. In this work, a novel approach to rapidly develop multi-material tissue-mimicking, cell-compatible platforms able to represent the complexity of a solid tumour's neo-vasculature is investigated via stereolithography three-dimensional printing. To do so, a series of acrylate resins that yield covalently photo-cross-linked hydrogels with healthy and diseased mechano-acoustic tissue-mimicking properties are designed and characterized. The potential viability of these materials to displace animal testing in preclinical research is assessed by studying the morphology, actin expression, focal adhesions and nitric oxide release of human umbilical vein endothelial cells. These materials are exploited to produce a simplified multi-material three-dimensional printed model of the neo-vasculature of a solid tumour, demonstrating the potential of our approach to replicate the complexity of solid tumours in vitro without the need for animal testing.
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影响因子：
7.2
作者：
Geiger B;Yamada KM
通讯作者：
Yamada KM
影响因子：
5
作者：
Goliszek, Marta;Podkoscielna, Beata;Sevastyanova, Olena
通讯作者：
Sevastyanova, Olena
影响因子：
1.4
作者：
Benjamin, Aaron D.;Abbasi, Reha;Wilking, James N.
通讯作者：
Wilking, James N.
DOI：
10.1126/science.aav9750
发表时间：
2019-05-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Grigoryan B;Paulsen SJ;Corbett DC;Sazer DW;Fortin CL;Zaita AJ;Greenfield PT;Calafat NJ;Gounley JP;Ta AH;Johansson F;Randles A;Rosenkrantz JE;Louis-Rosenberg JD;Galie PA;Stevens KR;Miller JS
通讯作者：
Miller JS
影响因子：
4.3
作者：
Cox TR;Erler JT
通讯作者：
Erler JT