A maturation protein in adenovirus morphogenesis.
A maturation protein in adenovirus morphogenesis.
复制标题
腺病毒形态发生中的成熟蛋白。
DOI：
10.1016/0042-6822(79)90287-3
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发表时间：
1979
期刊：
影响因子：
3.7
通讯作者：
U. Pettersson
中科院分区：
文献类型：
--
作者：
H. Persson;B. Mathisen;L. Philipson;U. Pettersson
用蔗糖梯度离心法分离腺病毒2型(AD2)组装中间体。这种中间体不同于成熟的病毒粒子，因为存在多肽VI、VII和VIII的未切割前体以及在成熟过程中消失的50,000(50K)的显著多肽(B.Edvardsson，E.Everitt，H.Jörnvall，L.Prange和L.Philipson，1976，J.Viroll.19,533-547；J.-C.D‘Halluin，G.Martin，G.Torpier和P.Boulanger，1978，J.Viro.26,357-363)。在目前的交流中，我们证明了50K多肽是由病毒基因组编码的，其基因位于片段SmaI-F的L链上(MAP坐标11.3T18.1)。
Adenovirus type 2 (ad2) assembly intermediates have been isolated by sucrose-gradient centrifugation. Such intermediates are distinguished from mature virions by the presence of uncleaved precursors to polypeptides VI, VII, and VIII as well as a prominent polypeptide with a molecular weight of 50,000 (50K) which disappears during maturation (B. Edvardsson, E. Everitt, H. Jörnvall, L. Prage, and L. Philipson, 1976,J. Virol.19, 533–547; J.-C. D'Halluin, G. Martin, G. Torpier, and P. Boulanger, 1978,J. Virol.26, 357–363). In the present communication we demonstrate that the 50K polypeptide is encoded by the viral genome and its gene is located on the l-strand of fragmentSmaI-F (map coordinates 11.3 to 18.1).