THE CONTROL OF TRANSFERRIN RECEPTOR SYNTHESIS IN MITOGEN-STIMULATED HUMAN-LYMPHOCYTES
THE CONTROL OF TRANSFERRIN RECEPTOR SYNTHESIS IN MITOGEN-STIMULATED HUMAN-LYMPHOCYTES
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DOI：
10.1016/0014-4827(84)90175-7
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发表时间：
1984-01-01
影响因子：
3.7
通讯作者：
LENNOX, ES
中科院分区：
文献类型：
--
作者：
PAUZA, CD;BLEIL, JD;LENNOX, ES
在植物促有丝分裂凝集素植物血凝素（PHA）的存在下培养的人淋巴细胞被激活并离开细胞周期的G0期。在PHA和原位产生的淋巴因子的存在下，细胞将进入S期并进行细胞分裂。转铁蛋白受体的外观的时间过程被确定为一个初步的尝试，以了解调节淋巴细胞分化和增殖的发病在48小时后加入PHA的分子机制。使用3种不同的测定方法，我们已经表明，表面受体分子数量的增加是由于新合成的受体的积累，而不是重新分配先前存在的受体分子库。转铁蛋白受体的总量增加至少4倍。在体外翻译的RNA从活化的淋巴细胞表明，新的受体合成是由于，至少部分地，增加的mRNA编码转铁蛋白受体的可用性。
Human lymphocytes cultured in the presence of the plant mitogenic lectin phytohemagglutinin in (PHA) become activated and leave the G0 phase of the cell cycle. In the presence of PHA and lymphokines produced in situ the cells will enter S phase and undergo cell division. The time course of appearance for the receptor for transferrin was determined as an initial attempt to understand the molecular mechanisms regulating the onset of lymphocyte differentiation and proliferation in the 48 h following PHA addition. Using 3 different assay methods we have shown that the increase in the number of surface receptor molecules is due to the accumulation of newly synthesized receptor and not to the redistribution of a previously existing pool of receptor molecules. The total amount of transferrin receptor increased at least 4-fold. In vitro translation of RNA from activated lymphocytes indicates that the new receptor synthesis is due, at least in part, to increased availability of mRNA encoding the transferrin receptor.