Proteomic analysis of haem-binding protein from Arabidopsis thaliana and Cyanidioschyzon merolae
Proteomic analysis of haem-binding protein from Arabidopsis thaliana and Cyanidioschyzon merolae
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DOI：
10.1098/rstb.2019.0488
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发表时间：
2020-05
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通讯作者：
Takayuki Shimizu;Rintaro Yasuda;Yui Mukai;Ryo Tanoue;T. Shimada;S. Imamura;Kan Tanaka;Satoru Watanabe;T. Masuda
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作者：
Takayuki Shimizu;Rintaro Yasuda;Yui Mukai;Ryo Tanoue;T. Shimada;S. Imamura;Kan Tanaka;Satoru Watanabe;T. Masuda
叶绿体生物发生涉及质体和核基因组的协调表达，需要从核向发育中的叶绿体发送信息，反之亦然。虽然细胞核如何控制叶绿体的发育是众所周知的，但对质体如何与细胞核通讯仍然知之甚少。目前，血红素被认为是一种逆行信号，参与植物和藻类光合作用相关的核基因表达和细胞周期等多种生理调控。然而，依赖于血红素的信号传导的成分仍然是未知的。在这项研究中，通过使用血红素固定的高性能亲和珠，我们进行了蛋白质组学分析的血红素结合蛋白，从拟南芥和Cyanidioschyzon merolae。大多数被鉴定的蛋白质是定位于各种细胞器中的非典型血蛋白。有趣的是，鉴定的蛋白质中有一半是核蛋白，其中一些在任一种或两种生物中具有相似的功能或定位。选择性蛋白质的生化分析表明血红素结合。本研究首次证实了植物和藻类的核蛋白具有血红素结合特性。这篇文章是“内共生细胞器的逆行信号”主题的一部分。
Chloroplast biogenesis involves the coordinated expression of the plastid and nuclear genomes, requiring information to be sent from the nucleus to the developing chloroplasts and vice versa. Although it is well known how the nucleus controls chloroplast development, it is still poorly understood how the plastid communicates with the nucleus. Currently, haem is proposed as a plastid-to-nucleus (retrograde) signal that is involved in various physiological regulations, such as photosynthesis-associated nuclear genes expression and cell cycle in plants and algae. However, components that transduce haem-dependent signalling are still unidentified. In this study, by using haem-immobilized high-performance affinity beads, we performed proteomic analysis of haem-binding proteins from Arabidopsis thaliana and Cyanidioschyzon merolae. Most of the identified proteins were non-canonical haemoproteins localized in various organelles. Interestingly, half of the identified proteins were nucleus proteins, some of them have a similar function or localization in either or both organisms. Following biochemical analysis of selective proteins demonstrated haem binding. This study firstly demonstrates that nucleus proteins in plant and algae show haem-binding properties. This article is part of the theme issue ‘Retrograde signalling from endosymbiotic organelles’.